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Proteomic analysis of liver tissue between fat and lean broiler lines
British Poultry Science ( IF 1.6 ) Pub Date : 2021-01-11 , DOI: 10.1080/00071668.2020.1847253
C. Wu 1, 2, 3 , C. Chen 1, 2, 3 , Y. Liu 1, 2, 3 , H. Li 1, 2, 3 , B. Cheng 1, 2, 3
Affiliation  

ABSTRACT

1. The liver is the major site of fatty acid synthesis in chickens. Lipid metabolism in the liver correlates with the deposition of triglycerides in adipose tissue. Northeast Agricultural University broilers lines divergently selected for abdominal fat content (NEAUHLF) provide a unique model to study the mechanisms of fat deposition.

2. In previous studies, differentially expressed genes (DEGs) in the livers of fat and lean broilers were evaluated across different developmental stages. Whether protein expression differences exist between the livers of fat and lean broilers is largely unknown. The current experiment used 2D fluorescence difference gel electrophoresis (2D-DIGE) to screen expressed protein (DEP) spots in the liver tissues of NEAUHLF at one, four and seven weeks of age.

3. Twenty-two DEPs were identified by MALDI-TOF-MS that were involved in lipid, energy, protein and amino acid metabolism, oxidative stress, cytoskeleton, and transport.

4. These data furthered the understanding of the fat and lean phenotypes of broiler chickens.



中文翻译:

脂肪和瘦肉鸡品系之间肝组织的蛋白质组学分析

抽象的

1.肝脏是鸡中脂肪酸合成的主要部位。肝脏中的脂质代谢与甘油三酸酯在脂肪组织中的沉积有关。东北农业大学针对腹部脂肪含量(NEAUHLF)选择不同的肉鸡生产线为研究脂肪沉积的机理提供了独特的模型。

2.在以前的研究中,在不同发育阶段评估了肥肉鸡和瘦肉鸡肝脏中的差异表达基因(DEG)。在肥肉鸡和瘦肉鸡的肝脏之间是否存在蛋白质表达差异尚不清楚。目前的实验使用2D荧光差异凝胶电泳(2D-DIGE)筛选NEAUHLF肝组织在1、4和7周龄时表达的蛋白(DEP)点。

3.通过MALDI-TOF-MS鉴定了22种DEP,涉及脂质,能量,蛋白质和氨基酸代谢,氧化应激,细胞骨架和运输。

4.这些数据进一步了解了肉鸡的脂肪和瘦肉表型。

更新日期:2021-02-26
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