To cope with soil contamination by trace elements (TE), phytomanagement has attracted much attention as being an eco-friendly and cost-effective green approach. In this context, aromatic plants could represent a good option not only to immobilize TE, but also to use their biomass to extract essential oils, resulting in high added-value products suitable for non-food valorization. However, the influence of aromatic plants cultivation on the bacterial community structure and functioning in the rhizosphere microbiota remains unknown. Thus, the present study aims at determining in TE-aged contaminated soil (Pb – 394 ppm, Zn – 443 ppm, and Cd – 7ppm, respectively, 11, 6, and 17 times higher than the ordinary amounts in regional agricultural soils) the effects of perennial clary sage (Salvia sclarea L.) cultivation, during two successive years of growth and inoculated with arbuscular mycorrhizal fungi, on rhizosphere bacterial diversity and community structure. Illumina MiSeq amplicon sequencing targeting bacterial 16S rRNA gene was used to assess bacterial diversity and community structure changes. Bioinformatic analysis of sequencing datasets resulted in 4691 and 2728 bacterial Amplicon Sequence Variants (ASVs) in soil and root biotopes, respectively. Our findings have shown that the cultivation of clary sage displayed a significant year-to-year effect, on both bacterial richness and community structures. We found that the abundance of plant-growth promoting rhizobacteria significantly increased in roots during the second growing season. However, we didn’t observe any significant effect of mycorrhizal inoculation neither on bacterial diversity nor on community structure. Our study brings new evidence in TE-contaminated areas of the effect of a vegetation cover with clary sage cultivation on the microbial soil functioning.

为了应对土壤微量元素（TE）污染，植物管理作为一种生态友好且具有成本效益的绿色方法而备受关注。在这种情况下，芳香植物不仅可以作为固定TE的一个很好的选择，而且还可以利用其生物质来提取精油，从而产生适合非食品增值的高附加值产品。然而，芳香植物栽培对根际微生物群细菌群落结构和功能的影响仍不清楚。因此，本研究旨在确定 TE 老化污染土壤（Pb – 394 ppm、Zn – 443 ppm 和 Cd – 7 ppm，分别比区域农业土壤中正常含量高 11、6 和 17 倍）的多年生快乐鼠尾草的功效（鼠尾草L.) 培养，在连续两年的生长过程中并接种丛枝菌根真菌，观察根际细菌多样性和群落结构。使用针对细菌 16S rRNA 基因的 Illumina MiSeq 扩增子测序来评估细菌多样性和群落结构变化。对测序数据集进行生物信息分析，在土壤和根生境中分别产生了 4691 个和 2728 个细菌扩增子序列变异体 (ASV)。我们的研究结果表明，快乐鼠尾草的种植对细菌丰富度和群落结构均表现出显着的逐年影响。我们发现，在第二个生长季节，根部促进植物生长的根际细菌的丰度显着增加。然而，我们没有观察到菌根接种对细菌多样性和群落结构有任何显着影响。 我们的研究为受 TE 污染的地区提供了新的证据，证明种植快乐鼠尾草的植被覆盖对微生物土壤功能的影响。