Effects of injured and dead cells of Escherichia coli on the colony‐forming rate of live cells,FEBS Open Bio

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Effects of injured and dead cells of Escherichia coli on the colony‐forming rate of live cells
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-12-02 , DOI: 10.1002/2211-5463.13051
Mikako Saito ₁ , Norimasa Takatani ₁ , Tomonori Yoshida ₁ , Alvin Mariogani ₁ , Eol Cho ₁ , Hideaki Matsuoka ₁

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Osmotic stress‐induced injured cells of Escherichia coli were prepared by sorting live cells onto tryptic soy agar (TSA) containing 10–50% sucrose. The time course of colony‐forming rate (CFR%) was analyzed. A time delay in colony formation indicated a sublethal effect. The final CFR level at 24 h indicated the relative number of culturable cells irrespective of injury. A value of (100‐CFR)% at 24 h indicated a lethal effect. When cells were grown on TSA containing 10% sucrose, the time delay was 4 h and the lethal effect was 4%. However, dead cells inhibited the growth of live cells. Physical contact with insoluble matter derived from dead cells or dead cells themselves might have caused growth inhibition. These findings highlight a novel perspective on colony count methods in practical situations, such as when sampling foods containing a high concentration of sucrose.

中文翻译：

大肠杆菌损伤和死细胞对活细胞集落形成率的影响

渗透胁迫诱导的大肠杆菌损伤细胞通过将活细胞分选到含有 10-50% 蔗糖的胰蛋白酶大豆琼脂 (TSA) 上来制备。分析集落形成率（CFR%）的时间过程。菌落形成的时间延迟表明存在亚致死效应。24 小时的最终 CFR 水平表示可培养细胞的相对数量，与损伤无关。24 小时时 (100-CFR)% 的值表示致死效应。当细胞在含有 10% 蔗糖的 TSA 上生长时，时间延迟为 4 h，致死率为 4%。然而，死细胞抑制了活细胞的生长。与来自死细胞或死细胞本身的不溶性物质的物理接触可能会导致生长抑制。这些发现突出了在实际情况下菌落计数方法的新观点，例如在对含有高浓度蔗糖的食物进行取样时。

更新日期：2020-12-02

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