AtNSF regulates leaf serration by modulating intracellular trafficking of PIN1 in Arabidopsis thaliana,Journal of Integrative Plant Biology

当前位置： X-MOL 学术 › J. Integr. Plant Biol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

AtNSF regulates leaf serration by modulating intracellular trafficking of PIN1 in Arabidopsis thaliana
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-12-02 , DOI: 10.1111/jipb.13043
Li Ping Tang _{1,

2} , Yi Yang _{1,

2} , Hui Wang ₃ , Lixin Li ₄ , Le Liu ₃ , Yu Liu _{1,

2} , Jinfeng Yuan _{1,

2} , Xiang Yu Zhao _{1,

2} , Klaus Palme _{2,

3,

5,

6} , Ying Hua Su _{1,

2} , Xugang Li _{1,

2}

Affiliation

In eukaryotes, N‐ethylmaleimide‐sensitive factor (NSF) is a conserved AAA+ATPase and a key component of the membrane trafficking machinery that promotes the fusion of secretory vesicles with target membranes. Here, we demonstrate that the Arabidopsis thaliana genome contains a single copy of NSF, AtNSF, which plays an essential role in the regulation of leaf serration. The AtNSF knock‐down mutant, atnsf‐1, exhibited more serrations in the leaf margin. Moreover, polar localization of the PIN‐FORMED1 (PIN1) auxin efflux transporter was diffuse around the margins of atnsf‐1 leaves and root growth was inhibited in the atnsf‐1 mutant. More PIN1‐GFP accumulated in the intracellular compartments of atnsf‐1 plants, suggesting that AtNSF is required for intracellular trafficking of PIN between the endosome and plasma membrane. Furthermore, the serration phenotype was suppressed in the atnsf‐1 pin1‐8 double mutant, suggesting that AtNSF is required for PIN1‐mediated polar auxin transport to regulate leaf serration. The CUP‐SHAPED COTYLEDON2 (CUC2) transcription factor gene is up‐regulated in atnsf‐1 plants and the cuc2‐3 single mutant exhibits smooth leaf margins, demonstrating that AtNSF also functions in the CUC2 pathway. Our results reveal that AtNSF regulates the PIN1‐generated auxin maxima with a CUC2‐mediated feedback loop to control leaf serration.

中文翻译：

AtNSF 通过调节拟南芥细胞内 PIN1 运输来调节叶片锯齿

在真核生物中， N-乙基马来酰亚胺敏感因子 (NSF) 是一种保守的 AAA+ATP 酶，是促进分泌囊泡与目标膜融合的膜运输机制的关键组成部分。在这里，我们证明拟南芥基因组包含一个NSF AtNSF 单拷贝，它在叶片锯齿的调节中发挥着重要作用。 AtNSF敲低突变体atnsf-1在叶缘表现出更多锯齿。此外，PIN-FORMED1 (PIN1) 生长素外流转运蛋白的极性定位在atnsf-1叶片边缘扩散，并且atnsf-1突变体的根生长受到抑制。更多的 PIN1-GFP 积累在atnsf-1植物的细胞内区室中，表明 AtNSF 是 PIN 在内体和质膜之间的细胞内运输所必需的。此外，锯齿表型在atnsf-1 pin1-8双突变体中受到抑制，表明 AtNSF 是 PIN1 介导的极性生长素转运调节叶片锯齿所必需的。 CUP-SHAPED COTYLEDON2 ( CUC2 ) 转录因子基因在atnsf-1植物中上调，并且cuc2-3单突变体表现出光滑的叶缘，表明 AtNSF 也在 CUC2 途径中发挥作用。我们的结果表明，AtNSF 通过 CUC2 介导的反馈回路调节 PIN1 生成的生长素最大值，以控制叶片锯齿。

更新日期：2020-12-02

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11