Soil bacteria play an essential role in functioning of ecosystems and maintaining of biogeochemical cycles. However, little is known about changes in the compositions and functional groups of soil bacterial communities during different restoration stages. The influences of aboveground vegetation and belowground soil properties on soil bacterial communities were also unclear during this process. Here we sequenced the soil bacterial communities in different stages of sand fixation. Sand fixation increased the diversity of the bacterial communities, among which the populations of Actinobacteria, Acidobacteria and Gemmatimonadetes changed significantly. The function prediction showed sand fixation increased Gram-positive and aerobic bacteria. Bacterial structure is significantly correlated with plant richness, coverage and biomass. In particular, we found species identity was an important determinant in structuring bacterial composition. Soil properties were all significantly correlated with soil bacterial community richness and diversity. Fusobacteria was strongly positively correlated with sand, Chloroflexi with total N and Gemmatimonadetes with SOM and total C. It suggested that soil nutrients (TC, TN and SOM) have large consequences for soil bacterial community dissimilarities. These results indicated that vegetation richness, especially species identity, together with improvement in soil nutrients, play key roles in driving the shifts in soil microbial community structure and function during restoration process.

中文翻译：

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植被丰富度、物种特性和土壤养分在恢复过程中驱动土壤细菌群落的变化

土壤细菌在生态系统的功能和生物地球化学循环的维持中起着至关重要的作用。然而，关于不同恢复阶段土壤细菌群落组成和功能组的变化知之甚少。在此过程中，地上植被和地下土壤性质对土壤细菌群落的影响也不清楚。在这里，我们对固沙不同阶段的土壤细菌群落进行了测序。固沙增加了细菌群落的多样性，其中放线菌、酸杆菌和Gemmatimonadetes的种群发生了显着变化。功能预测显示固沙增加了革兰氏阳性菌和好氧菌。细菌结构与植物丰富度、覆盖率和生物量显着相关。特别是，我们发现物种身份是构建细菌组成的重要决定因素。土壤性质均与土壤细菌群落丰富度和多样性显着相关。Fusobacteria与沙子、 Chloroflexi与总氮和Gemmatimonadetes呈强正相关与 SOM 和总 C。这表明土壤养分 (TC、TN 和 SOM) 对土壤细菌群落差异有很大影响。这些结果表明，植被丰富度，特别是物种同一性，以及土壤养分的改善，在驱动恢复过程中土壤微生物群落结构和功能的变化中起着关键作用。