The Expansin protein is known for its multifaceted roles in plant growth, especially cell walls. However, very few studies have been done so far to assess the effects of expansin genes on Cotton fibre development. The present study is a successful effort to fill this gap, where the α‐EXPA1 gene transformed into a local cotton variety, Gossypium hirsutum, through Agrobacterium‐mediated transformation under Gossypium hirsutum Seed coat and Fibre‐specific promoter (GhSCFP). The transgenic cotton plants underwent molecular characterization and fibre trait evaluation. Our results indicated that α‐EXPA1 showed an up‐regulated expression during the transition phase of secondary cell wall synthesis and resulted in improving the fibre parameters, especially micronaire value. Transgenic cotton fibre also showed a finer twisting under the Scanning electron microscope (SEM) as compared to non‐transgenic cotton fibre samples. The fibre production is influenced by more than nine thousand genes, and the fibre improvement cannot be just achieved through a single gene transformation. However, α‐EXPA1 is one of the potential candidates for cotton fibre research as it significantly improved the cotton fibre.

中文翻译：

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α‐EXPA1基因的转化导致陆地棉的纤维质量提高

Expansin蛋白以其在植物生长特别是细胞壁中的多方面作用而闻名。但是，到目前为止，很少有研究评估弹性蛋白基因对棉纤维发育的影响。本研究是一个成功的努力来填补这一空白，其中该α- EXPA1基因转化到本地棉花品种，陆地棉下，通过农杆菌介导的转化陆地棉种皮和纤维特异性启动子（GhSCFP） 。对转基因棉花进行了分子鉴定和纤维性状评估。我们的结果表明α‐ EXPA1在次级细胞壁合成的过渡阶段显示出上调的表达，并改善了纤维参数，尤其是马克隆值。与非转基因棉纤维样品相比，转基因棉纤维在扫描电子显微镜（SEM）的作用下也表现出更好的扭曲。纤维生产受到超过九千个基因的影响，而纤维改良不能仅通过单基因转化来实现。但是，α‐EXPA1是棉纤维研究的潜在候选者之一，因为它显着改善了棉纤维。