Isolation and characterization of twelve polymorphic microsatellite markers in the endangered Hopea hainanensis (Dipterocarpaceae),Ecology and Evolution

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Isolation and characterization of twelve polymorphic microsatellite markers in the endangered Hopea hainanensis (Dipterocarpaceae)
Ecology and Evolution ( IF 2.3 ) Pub Date : 2020-12-02 , DOI: 10.1002/ece3.7077
Chen Wang ₁ , Xiang Ma ₁ , Liang Tang ₂

Affiliation

Microsatellite markers were isolated and characterized for Hopea hainanensis Merrill & Chun, an endangered tree species with scattered distribution in Hainan Island and northern Vietnam. Twenty‐six microsatellite markers were developed based on next‐generation sequencing data and were genotyped by capillary electrophoresis on an ABI 3730xl DNA Analyzer. Twelve markers were found to be polymorphic in H. hainanensis. GENODIVE analyses indicated that the number of alleles ranged from 2 to 6 per locus, and the observed and expected heterozygosity varied from 0 to 0.755 and from 0.259 to 0.779, respectively. Primer transferability was tested with Hopea chinensis Hand.‐Mazz. and Hopea reticulata Tardieu, in which 3 and 7 microsatellite markers were found to be polymorphic, separately. The results showed that H. reticulata and H. hainanensis had similar levels of genetic diversity. A neighbor joining dendrogram clustered all individuals into two major groups, one of which was exclusively constituted by H. hainanensis, while the other consisted of two subgroups, corresponding to H. reticulata and H. chinensis, respectively. The 12 polymorphic microsatellite markers could be applied to study genetic diversity, population differentiation, mating system, and fine‐scale spatial genetic structures of H. hainanensis as well as its close relatives, facilitating the conservation and restoration of these endangered but valuable Hopea species.

中文翻译：

濒危龙脑香科12个多态性微卫星标记的分离与鉴定

对分散分布于海南岛和越南北部的濒危树种海南希望树进行了微卫星标记的分离和表征。基于下一代测序数据开发了 26 个微卫星标记，并在 ABI 3730xl DNA 分析仪上通过毛细管电泳进行基因分型。在海南花中发现了12个多态性标记。 GENODIVE 分析表明，每个位点的等位基因数量为 2 至 6 个，观察到的杂合度和预期杂合度分别为 0 至 0.755 和 0.259 至 0.779。使用Hopea chinensis Hand.‐Mazz 测试了底漆的可转移性。和Hopea reticulata Tardieu，其中分别发现3个和7个微卫星标记是多态性的。结果表明， H. reticulata和H. hainanensis具有相似的遗传多样性水平。邻接树状图将所有个体聚类为两个主要类群，其中一个仅由H. hainanensis组成，而另一个则由两个亚类组成，分别对应于H. reticulata和H. chinensis 。 12个多态性微卫星标记可用于研究海南花及其近缘植物的遗传多样性、种群分化、交配系统和精细空间遗传结构，促进这些濒危但有价值的希望树物种的保护和恢复。

更新日期：2021-01-08

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