A Spectroscopic Approach towards the Comparative Binding Studies of the Antioxidizing Flavonol Myricetin with Various Single‐Stranded RNA,ChemistrySelect

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A Spectroscopic Approach towards the Comparative Binding Studies of the Antioxidizing Flavonol Myricetin with Various Single‐Stranded RNA
ChemistrySelect ( IF 1.9 ) Pub Date : 2020-12-02 , DOI: 10.1002/slct.202003601
Susmita Chowdhury ₁ , Sutanwi Bhuiya ₁ , Lucy Haque ₁ , Suman Das ₁

Affiliation

RNA based drugs have come to limelight owing to the major role played by RNA in the development of many viral diseases. Molecules which can effectively bind with RNA can induce morphological changes in RNA which further modifies its biological properties. Flavonoids are phytochemicals having a wide range of pharmacological properties and are known to interact with DNA. We have used RNA instead of DNA and aimed at establishing a comparison of binding of three single stranded forms of RNA homopolymers namely polyriboadenylic acid [poly(rA)], polyuridylic acid [poly(rU)] and polycytidylic acid [poly(rC)] with the flavonol myricetin (herein after MTN), our probe of interest. A series of spectroscopic experiments like spectrophotometry, spectrofluorimetry and circular dichroism studies indicate a stronger binding of myricetin with poly(rA) followed by poly(rU) and finally poly(rC). This was confirmed from the construction of Scatchard plot using the Mc‐Ghee von Hippel equation for cooperative binding. The magnitude of the order of binding was 10³ M⁻¹ at 25 °C in all the cases. It is to be noted that most eukaryotic mRNAs have a poly adenylate tail. Binding of MTN to poly(rA) in virulent cells could effectively alter the encoded protein synthesis and reduce chances of occurrence of the disease.

中文翻译：

用光谱学方法比较抗氧化黄酮醇杨梅素与各种单链RNA的结合研究

由于RNA在许多病毒性疾病的发展中起着重要作用，因此基于RNA的药物备受关注。可以有效结合RNA的分子可以诱导RNA的形态变化，从而进一步修饰其生物学特性。类黄酮是具有广泛药理特性的植物化学物质，已知与DNA相互作用。我们使用RNA代替DNA，旨在建立三种单链形式的RNA均聚物，即聚核糖腺苷酸[poly（rA）]，聚尿苷酸[poly（rU）]和聚胞苷酸[poly（rC）]的结合比较。黄酮杨梅素（以下简称MTN）是我们的研究对象。一系列分光镜实验，例如分光光度法，光谱荧光法和圆二色性研究表明，杨梅素与poly（rA），随后是poly（rU），最后是poly（rC）的结合力更强。这是通过使用Mc-Ghee von Hippel方程进行合作绑定的Scatchard图的构建得到证实的。绑定顺序的大小为10在所有情况下，在25°C下为³ M ^-1。应当指出，大多数真核mRNA具有聚腺苷酸尾。MTN与毒性细胞中的poly（rA）结合可有效改变编码的蛋白质合成，并减少疾病发生的机会。

更新日期：2020-12-02

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