Purification and Identification of Pine Nut (Pinus yunnanensis Franch.) Protein Hydrolysate and its Antioxidant Activity in Vitro and in Vivo,Chemistry & Biodiversity

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Purification and Identification of Pine Nut (Pinus yunnanensis Franch.) Protein Hydrolysate and its Antioxidant Activity in Vitro and in Vivo
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2020-12-22 , DOI: 10.1002/cbdv.202000710
Zitian Liu ₁ , Yuming Shi ₁ , Huiping Liu ₁ , Qi Jia ₁ , Qingrun Liu ₁ , Jianqiu Tu ₁

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In this study, the pine nut (Pinus yunnanensis Franch.) protein was hydrolyzed by alkaline protease and trypsin to prepare pine nut protein hydrolysate (PNPH). The chemical, intracellular and in vivo antioxidant capacity of PNPH were evaluated. PNPH owned the ability of scavenging free radicals, and it could protect the HepG2 cells from oxidative damage by preserving cell viability. Moreover, PNPH could reduce the malondialdehyde (MDA) content and improved the superoxide dismutase (SOD) and glutathione peroxidase (GSH‐Px) activities in serum, heart and liver of aging mice induced by D‐galactose. Further, the PNPH was stepwise purified and identified, and 15 peptides were identified from purified fraction in PNPH. The three‐dimension structures of identified peptides were predicted. Among all identified peptides, peptide 3, 7, 8 and 11 were presumed to possess good antioxidant activity. Overall, PNPH and purified peptides isolated from PNPH have potential application prospects in the field of natural antioxidants and anti‐aging functional foods.

中文翻译：

松子（Pinus yunnanensis Franch.）蛋白水解物的纯化和鉴定及其体外和体内抗氧化活性

本研究采用碱性蛋白酶和胰蛋白酶水解松子（Pinus yunnanensis Franch.）蛋白制备松子蛋白水解物（PNPH）。评估了 PNPH 的化学、细胞内和体内抗氧化能力。PNPH具有清除自由基的能力，可以通过保持细胞活力来保护HepG2细胞免受氧化损伤。此外，PNPH可以降低丙二醛（MDA）含量，提高D-半乳糖诱导的衰老小鼠血清、心脏和肝脏中的超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性。进一步对PNPH进行了逐步纯化和鉴定，从PNPH中纯化的级分中鉴定出15个肽段。预测了鉴定肽的三维结构。在所有鉴定的肽中，肽 3、7、推测8和11具有良好的抗氧化活性。总的来说，PNPH和从PNPH中分离的纯化肽在天然抗氧化剂和抗衰老功能食品领域具有潜在的应用前景。

更新日期：2020-12-22

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