Effluent containing phenol is hazardous wastewater for the environment and human health. In the current work, synthesis and characterization of a new bi-enzymatic, magnetic-core nanocatalyst was investigated, and its performance as nanocatalyst for phenol removal from wastewater was examined. The obtained data suggested that the maximum combination of Peroxidases (POD) and Polyphenol Oxidase (PPO) enzymes (CPPE) stabilization can be achieved at T=20 °C, pH value of 8, glutehydrie concentration of 1.6%, and enzyme to nanoparticles ratio of 1:200. Furthermore, it was found that the immobilized enzymes had higher stability than free enzyme against change in pH, temperature and even their durability. The Immobilized enzymes were used for phenol removal, and 98.1% separation was observed after 145 min when H₂O₂ to phenol ratio was 1.2, and in high concentrations of phenol (in the range of 2 to 10 mM) in the presence of CPPE. Finally, the stabilized enzymes were used 7 times in the phenol removal process, and the enzymes retained about 50% of their initial activity after 6 uses. It seems that the CPPE-alginate nanocatalyst could be used in hazardous wastewater treatment in particular for phenol removal.

含有苯酚的废水是对环境和人类健康有害的废水。在当前的工作中，研究了一种新型的双酶，磁芯纳米催化剂的合成和表征，并研究了其作为用于去除废水中苯酚的纳米催化剂的性能。获得的数据表明，过氧化物酶（POD）和多酚氧化酶（PPO）酶（CPPE）稳定化的最大组合可以在T = 20°C，pH值为8，糖水浓度为1.6％和酶与纳米颗粒比率的情况下实现1：200。此外，发现固定化酶具有比游离酶更高的针对pH，温度甚至其耐久性的稳定性。固定化酶用于去除苯酚，H ₂ O 145分钟后分离率达到98.1％在CPPE存在下，苯酚的₂比为1.2，高浓度的苯酚（在2至10 mM范围内）。最后，稳定的酶在苯酚去除过程中使用了7次，使用6次后，这些酶保留了其初始活性的50％。似乎CPPE-藻酸盐纳米催化剂可用于危险废水处理，特别是用于去除苯酚。