ZnO nanoparticles were synthesized by green synthesis using Cannabis (GSZ-1), Jatropha curcas (GSZ-2), Alovera(GSZ-3),and Tinosporacordifolia(GSZ-4) plant and leaves extract. Phase formation of ZnO nanoparticles were analyzed by X-Ray Diffraction and Rietveld Refinement. FTIR study confirms the presence of secondary metabolites like phenols and terpenoids, polyphenols, phenolic, saponin, alkaloids and glycosides in plant leaves extract working as reducing agents to shapeup ZnO nanoparticles at room temperature. SEM images provide details of the effect of plant extracts on the growth mechanism of nanoparticles and surface morphology of synthesized GSZ-1, GSZ-2, GSZ-3, and GSZ-4. EDX spectroscopy displays the ionic distribution of Zn and O in the grain of the all synthesized samples. UV-Visible spectroscopy was performed to find out the optical properties of the synthesized ZnO nanoparticles. The test cultures included in this study were pathogenic gram-positive bacterial strains Basillus cereus (BCC4391), Staphylococcus aureus (SCC2993) and one Gram-negative bacteria strain Klebsiella pneumoniae (KCC3812).

使用大麻（GSZ-1），麻疯树（GSZ-2），Alovera（GSZ-3 ）和Tinosporacordifolia通过绿色合成法合成ZnO纳米颗粒（GSZ-4）植物和树叶提取物。通过X射线衍射和Rietveld精炼分析了ZnO纳米粒子的相形成。FTIR研究证实植物叶片提取物中存在次生代谢产物，如酚和萜类化合物，多酚，酚类，皂苷，生物碱和糖苷，可作为还原剂在室温下塑造ZnO纳米颗粒。SEM图像提供了植物提取物对纳米颗粒生长机理和合成的GSZ-1，GSZ-2，GSZ-3和GSZ-4的表面形态的影响的详细信息。EDX光谱显示所有合成样品的晶粒中Zn和O的离子分布。进行紫外可见光谱以发现合成的ZnO纳米颗粒的光学性质。这项研究中包括的测试培养物是致病性革兰氏阳性细菌菌株蜡状芽孢杆菌（BCC4391），金黄色葡萄球菌（SCC2993）和一种革兰氏阴性菌肺炎克雷伯菌（KCC3812）。