Organoids are self-organising stem cell-derived ex vivo cultures widely adopted as biomimetic models of healthy and diseased tissues. Traditional low-dimensional experimental methods such as microscopy and bulk molecular analysis have generated remarkable biological insights from organoids. However, as complex heterocellular systems, organoids are especially well-positioned to take advantage of emerging high-dimensional technologies. In particular, single-cell methods offer considerable opportunities to analyse organoids at unprecedented scale and depth, enabling comprehensive characterisation of cellular processes and spatial organisation underpinning organoid heterogeneity. This review evaluates state-of-the-art analytical methods applied to organoids, discusses the latest advances in single-cell technologies, and speculates on the integration of these two rapidly developing fields.

类器官是自组织干细胞衍生的离体培养物，被广泛用作健康和患病组织的仿生模型。传统的低维实验方法，例如显微镜和本体分子分析，已经从类器官中产生了显着的生物学见解。然而，作为复杂的异质细胞系统，类器官特别适合利用新兴的高维技术。特别是，单细胞方法为以前所未有的规模和深度分析类器官提供了相当多的机会，从而能够全面表征支持类器官异质性的细胞过程和空间组织。这篇综述评估了应用于类器官的最先进的分析方法，讨论了单细胞技术的最新进展，并推测了这两个快速发展领域的整合。