Major depressive disorder (MDD) is a prevalent psychiatric disorder that brings great harm and burden to both patients and society. This study aimed to examine the effects of whole-body vibration (WBV) training on a chronic restraint stress (CRS) induced depression rat model and provide an initial understanding of related molecular mechanisms. Adult Sprague-Dawley male rats were randomly divided into the following three groups: a) control group, b) depressive disorder group, and c) depression with WBV training treatment group. Daily food intake, body weight, sucrose preference test, open field test, elevated plus maze, forced swimming test, and Barnes maze task tests were performed. Immunofluorescence staining and ELISA analysis were used to assess neuronal damage, synaptic proteins, glial cells, and trophic factors. The data of behavioral tests and related biochemical indicators were statistically analyzed and compared between groups. Rats undergoing CRS showed increased anxiety-like behavior and memory impairment, along with synaptic atrophy and neuronal degeneration. WBV could reverse behavioral dysfunction, inhibit the degeneration of neurons, alleviate the damage of neurons and the pathological changes of glial cells, enhance trophic factor expression, and ameliorate the downregulation of dendritic and synaptic proteins after CRS. The effect of WBV in rats may be mediated via the reduction of hippocampal neuronal degeneration and by improving expression of synaptic proteins. WBV training exerts multifactorial benefits on MDD that supports its use as a promising new therapeutic option for improving depression-like behaviors in the depressive and/or potentially depressive.

重度抑郁症（MDD）是一种普遍存在的精神疾病，给患者和社会都带来了极大的伤害和负担。本研究旨在检查全身振动 (WBV) 训练对慢性束缚应激 (CRS) 诱导的抑郁大鼠模型的影响，并初步了解相关分子机制。成年 Sprague-Dawley 雄性大鼠随机分为以下三组：a) 对照组，b) 抑郁症组，c) WBV 训练抑郁症治疗组。进行每日食物摄入量、体重、蔗糖偏好测试、野外测试、高架十字迷宫、强迫游泳测试和巴恩斯迷宫任务测试。免疫荧光染色和 ELISA 分析用于评估神经元损伤、突触蛋白、神经胶质细胞和营养因子。对行为学检查数据及相关生化指标进行统计学分析和组间比较。接受 CRS 的大鼠表现出更多的焦虑样行为和记忆障碍，以及突触萎缩和神经元变性。WBV可以逆转行为功能障碍，抑制神经元变性，减轻神经元损伤和神经胶质细胞的病理变化，增强营养因子的表达，改善CRS后树突和突触蛋白的下调。WBV 在大鼠中的作用可能是通过减少海马神经元变性和提高突触蛋白的表达来介导的。