In recent years, scaffolds produced in 3D printing technology have become more widespread tool due to providing more advantages than traditional methods in tissue engineering applications. In this research, it was aimed to produce patches for the treatment of tympanic membrane perforations which caused significant hearing loss by using 3D printing method. Polylactic acid(PLA) scaffolds with Chitosan(CS) and Sodium Alginate(SA) added in various ratios were prepared for artificial eardrum patches. Different amounts of chitosan and sodium alginate added to PLA increased the biocompatibility of the produced scaffolds. The created patches were designed by mimicking the thickness of the natural tympanic membrane thanks to the precision provided by the 3D printed method. The produced scaffolds were analyzed separately for chemical, morphological, mechanical and biocompatibility properties. Scanning electron microscope (SEM), Fourier-transform infrared (FT-IR) spectroscopy was performed to observe the surface morphology and chemical structure of the scaffolds. Mechanical, thermal and physical properties, swelling and degradation behaviors were examined to fully analyze whole characteristic features of the samples. Cell culture study was also performed to demonstrate the biocompatibility properties of the fabricated scaffolds with human adipose tissue-derived mesenchymal stem cells (hAD-MSCs). 15 wt % PLA was selected as the control group and among all concentrations of CS and SA, groups containing 3 wt% CS and 3 wt% SA showed significantly superior and favorable features in printing quality. The research continued with these two scaffolds (3 wt% CS, and 3 wt% SA), which showed improved print quality when added to PLA. Overall, these results show that PLA/CS and PLA/SA 3D printed artificial patches have the potential to tissue engineering solutions to repair tympanic membrane perforation for people with hearing loss.

近年来，由于3D打印技术生产的支架在组织工程应用中比传统方法具有更多优势，因此已成为一种更广泛的工具。在这项研究中，旨在通过使用3D打印方法来生产用于治疗导致严重听力损失的鼓膜穿孔的贴剂。制备了以不同比例加入的壳聚糖（CS）和海藻酸钠（SA）的聚乳酸（PLA）支架，用于人工鼓膜贴剂。添加到PLA中的壳聚糖和藻酸钠的量不同，可以提高所生产支架的生物相容性。借助3D打印方法提供的精度，可以通过模仿天然鼓膜的厚度来设计制作的贴片。分别对产生的支架进行化学，形态，机械和生物相容性。进行扫描电子显微镜（SEM），傅立叶变换红外光谱（FT-IR）光谱观察支架的表面形态和化学结构。检查了机械，热和物理性能，溶胀和降解行为，以全面分析样品的整个特征。还进行了细胞培养研究，以证明所制备的支架与人脂肪组织来源的间充质干细胞（hAD-MSC）的生物相容性。选择15wt％的PLA作为对照组，并且在所有浓度的CS和SA中，包含3wt％的CS和3wt％的SA的组在印刷质量上显示出明显优越的和有利的特征。继续使用这两种支架（3 wt％CS和3 wt％SA）进行研究，当添加到PLA时，显示出改善的打印质量。总体而言，这些结果表明，PLA / CS和PLA / SA 3D打印的人工贴片具有组织工程解决方案的潜力，可以修复听力损失者的鼓膜穿孔。