Attempts to improve HPLC performance often focus on increasing the speed or separation performance. In this article, both the flow rate and column length are optimized as separation conditions, while observing the number of theoretical plates and hold-up time with isocratic elutions. In addition, the upper pressure limit must be simultaneously considered as the boundary condition. Approaches based on the optimal velocity (Opt.) are often adopted; but the kinetic performance limit (KPL) in Desmet's method can also be utilized for three-dimensional graphing with axes of pressure, time, and number of theoretical plates. Here, two approaches involving pressure increase are introduced, beginning with the condition of optimal linear velocity: one aimed at greater speed and the other at higher resolution. Coefficients of pressure-application are derived to measure the effectiveness of the intermediate conditions between the Opt. and KPL methods. In the third approach, the hold-up time is extended while maintaining a fixed pressure. Coefficients of time-extension are also derived, to determine the effectiveness to improve the separation performance.

尝试提高HPLC性能通常集中在提高速度或分离性能上。在本文中，流速和色谱柱长度均作为分离条件进行了优化，同时观察了理论板数和等度洗脱的保留时间。另外，必须同时考虑压力上限。通常采用基于最佳速度的方法。但是Desmet方法中的动力学性能极限（KPL）也可以用于压力，时间和理论塔板数的三维图形绘制。这里，介绍了两种涉及压力增加的方法，从最佳线速度的条件开始：一种针对更高的速度，另一种针对更高的分辨率。得出压力施加系数，以测量Opt之间的中间条件的有效性。和KPL方法。在第三种方法中，在保持固定压力的同时延长了保持时间。还得出时间延长系数，以确定改善分离性能的有效性。