Cast films obtained from polyvinyl alcohol (PVOH) blended with casein hydrolysates (HCas) in a weight ratio of 1:1 were employed to carry nisin-producing L. lactis and phytic acid in order to broaden the antimicrobial spectrum of L. lactis to Gram-positive and Gram-negative spoilage and pathogen bacteria. For this purpose, the effect of the antimicrobial activity of various film formulations and combinations of films on the growth of E. coli at 37 °C for 24 h was studied. The film system that showed antimicrobial activity against Gram-negative bacteria consisted of phytic acid and L. lactis incorporated in separate films. When the active agents were in the same film the viability of L. lactis decreased considerably and it did not exert antimicrobial activity against the bacterium. Therefore, the combination of L. lactis and phytic acid in separate films was chosen as the reliable system, and the effect of its activity on the growth of Gram-negative bacteria (E. coli, Salmonella enterica, and Pseudomonas fluorescens) and Gram-positive bacteria (Listeria monocytogenes) in liquid culture medium was tested at refrigeration temperature (4 °C), and with simulated breaks in the cold chain (14 °C and 24 °C). The survival of L. lactis in coexistence with these bacteria was also studied. The film system exerted an antimicrobial effect against the Gram-negative bacteria tested, and the activity depended on the bacteria and the temperature assayed. With regard to the antimicrobial activity against L. monocytogenes, phytic acid improved the antimicrobial capacity of L. lactis. The survival of L. lactis was maintained at 7–8 log (CFU/mL) culture in liquid medium throughout the storage period.

The films developed were intended to be used as coatings in the design of a double-sided active bag for a non-fermented dairy product. The bags were filled with homemade preservative-free pastry cream, and the microbiological shelf life and evolution of pH of the packaged ready-to-eat food stored at 4 °C was studied for 20 days. The results showed a reduction in the growth of spoilage bacteria and therefore an increase in the shelf life of the packaged product. The films developed could be applied in the design of packages for perishable dairy foods in order to increase their microbiological shelf life.

从聚乙烯醇得到的流延薄膜（PVOH）共混有酪蛋白水解的重量比为1（HCAS）：1被用于携带乳链菌肽产生大号。乳酸和植酸为了扩大L的抗菌谱。乳酸可导致革兰氏阳性和革兰氏阴性腐败菌和病原菌。为此，研究了各种薄膜制剂和薄膜组合的抗菌活性对大肠杆菌在37°C下生长24小时的影响。对革兰氏阴性菌具有抗菌活性的薄膜系统由植酸和L组成。乳酸菌合并在单独的电影中。当活性剂在同一膜中时，L的生存力。乳酸明显减少，对细菌没有抗菌活性。因此，L的组合。选择单独膜中的乳酸和植酸作为可靠的体系，并对其活性对革兰阴性菌（大肠杆菌，肠沙门氏菌和荧光假单胞菌）和革兰氏阳性细菌（单核细胞增生性李斯特菌）生长的影响。液体培养基在冷藏温度（4°C）和冷链模拟断裂（14°C和24°C）下进行了测试。生存之道大号。乳与这些细菌共存进行了研究。该膜系统对所测试的革兰氏阴性菌具有抗菌作用，其活性取决于所测细菌和温度。关于对L的抗菌活性。菌等，植酸改进的抗微生物能力L. 球菌。L的存活。球菌保持在7-8日志（CFU / mL）的培养在整个贮存期液体介质。

所开发的薄膜旨在用作非发酵乳制品双面活性包装袋设计中的涂层。这些袋子装满了自制的无防腐剂的面霜，并研究了在4°C下储存的即食食品的微生物货架期和pH值变化20天。结果显示变质细菌的生长减少，因此包装产品的货架期增加。所开发的薄膜可用于易腐乳制品包装的设计中，以延长其微生物货架寿命。