Nitrite-dependent anaerobic methane-oxidizing (n-damo) process has a promising prospect in anaerobic wastewater treatment, utilizing methane as the sole electron source to remove nitrite. However, the metabolic activity of n-damo bacteria is too low for practical application. This study aimed to stimulate n-damo process by introducing conductive nano-magnetite and/or electron shuttle anthraquinone-2,6-disulfonate (AQDS), and also set a comparative treatment of adding insulated ferrihydrite. The results showed that the nitrite reduction rate was enhanced the most significantly in treatment with nano-magnetite, approximately 1.6 times higher than that of the control without any supplement. While ferrihydrite application showed an adverse effect on n-damo process. The well-known aerobic methane oxidizer Methylomonas spp. was found to be enriched under n-damo condition with the supplementation of nano-magnetite and/or AQDS, but abundance of n-damo bacteria did not exhibit significant increase. It was hypothesized that Methylomonas spp. could be survived under anaerobic n-damo condition using oxygen produced by n-damo bacteria for the self-growth, and the nitrite reduction could be promoted through the enhancement of microbial interspecies electron transfer triggered by the introduction of conductive materials. It opens a new direction for the stimulation of n-damo activity, which needs more evidences to verify the hypothetic mechanism.

以亚硝酸盐为基础的厌氧甲烷氧化（n-damo）工艺在厌氧废水处理中具有广阔的前景，该技术利用甲烷作为唯一的电子源来去除亚硝酸盐。但是，n-damo细菌的代谢活性对于实际应用而言太低。这项研究旨在通过引入导电的纳米磁铁矿和/或电子穿梭蒽醌2,6-二磺酸盐（AQDS）来刺激n-damo过程，并且还提出了添加绝缘亚铁水合物的比较方法。结果表明，在使用纳米磁铁矿的处理中，亚硝酸盐的还原速率得到了最大的提高，比不添加任何添加剂的对照提高了约1.6倍。虽然使用水铁矿显示出对正达莫过程的不利影响。著名的有氧甲烷氧化甲基单spp。发现在n-damo条件下添加了纳米磁铁矿和/或AQDS可使其富集，但n-damo细菌的丰度并未显着增加。假设是甲基单孢菌属。可以在厌氧的n-damo条件下利用n-damo细菌产生的氧气进行自生，并通过引入导电材料触发微生物间的种间电子转移来促进亚硝酸盐的还原。它为刺激n-damo活性开辟了一个新的方向，需要更多的证据来验证这一假设机制。