Abstract A novel fabrication of polymer composite fibers using polycaprolactone (PCL), montmorillonite nanoclay (MMT-Clay), and nano-hydroxyapatite-clay (HAP MMT-Clay) is reported for bone tissue engineering applications. Using a pressurized gyration (PG) setup, polycaprolactone (PCL) fibers incorporated with in situ mineralized HAP MMT-Clay and MMT-Clay were investigated. Using the novel fabrication method, we were able to successfully manufacture HAP-nanoclay-PCL fibers. Further, 3D scaffolds made using the prepared fibers were able to enhance bone growth, cell viability, and proliferation. The results demonstrated that the polymer fiber scaffolds are biocompatible, and the cells were able to thrive and differentiate on the fiber scaffolds. A significant increase in cell viability, osteogenic differentiation, ECM formation, and collagen formation was observed with PCL HAP MMT-Clay fibers scaffolds compared to the behaviors in PCL fibers. Further, the intracellular ALP levels increased with PCL HAP MMT-Clay fiber scaffold, indicating enhanced osteogenic differentiation of MSCs. This work shows a promising outlook for the future of manufacturable composite nanoclay polymer fibers incorporated as scaffolds for bone tissue engineering applications.

中文翻译：

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使用加压旋转制造用于骨组织工程的复合纳米粘土-羟基磷灰石-聚合物纤维支架

摘要 使用聚己内酯 (PCL)、蒙脱石纳米粘土 (MMT-Clay) 和纳米羟基磷灰石-粘土 (HAP MMT-Clay) 制备的聚合物复合纤维用于骨组织工程应用。使用加压回转 (PG) 装置，研究了与原位矿化 HAP MMT-Clay 和 MMT-Clay 结合的聚己内酯 (PCL) 纤维。使用新的制造方法，我们能够成功制造 HAP-纳米粘土-PCL 纤维。此外，使用制备的纤维制成的 3D 支架能够增强骨骼生长、细胞活力和增殖。结果表明聚合物纤维支架具有生物相容性，细胞能够在纤维支架上茁壮成长和分化。显着增加细胞活力、成骨分化、ECM 形成、与 PCL 纤维中的行为相比，使用 PCL HAP MMT-Clay 纤维支架观察到胶原形成。此外，细胞内 ALP 水平随着 PCL HAP MMT-Clay 纤维支架的增加而增加，表明 MSC 的成骨分化增强。这项工作显示了可制造复合纳米粘土聚合物纤维作为骨组织工程应用支架的未来前景。