The heterogeneous lignocellulosic contents of oil palm empty fruit bunch (OPEFB) fiber requires the use of chemical or biological pre-treatment for further value-added applications. An effective biological pre-treatment was preferred since it is mild, low energy, substrate specific and will not generate any inhibitory chemicals that interfere enzymatic activities and fermentation process. The biological pre-treatment of OPEFB fibers by local isolate Pycnoporus sanguineus was investigated in this work to improve its biodegradability through laccase production via solid-state fermentation approach. The effects of physical parameters (temperature, inducer concentrations, substrate loading) were explored to obtain maximum laccase production by P. sanguines. Results showed that the use of extractive-free OPEFB fiber at 30 °C supplemented with 4 mM of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 10 g of OPEFB loading yielded laccase production that was 2.1 fold higher compared to raw OPEFB. The changes of chemical and surface structures of raw, chemicals and laccase treated OPEFB were reported from Fourier-transform infrared spectroscopy (FTIR), thermogravimetric (TGA) and scanning electron microscopy (SEM) analyses. The simulated techno-economic analysis suggested economic feasibility of a batch bio-refinery for laccase production in an industrial scale feed at a maximum rate of 50 tons OPEFB/batch. From the simulated baseline study, the cost price forecast for laccase production was 14.26 US$/kg owing to the capital (65.87%) and raw materials costs (31.68%). This study reveals the potential of P. sanguineus to be used not only in biorefinery industry but also in biopulping, biobleaching (in textile and paper industries) and degrading phenolic waste (dye, colorant, pesticide waste).

油棕空果束（OPEFB）纤维的异质木质纤维素含量需要进行化学或生物预处理，才能进一步实现增值应用。优选有效的生物预处理，因为它是温和的，低能量的，底物特异性的，并且不会产生任何干扰酶活性和发酵过程的抑制性化学物质。在这项工作中，研究了通过局部分离的碧萝P（Pycnoporus sanguineus）对OPEFB纤维进行的生物预处理，以通过固态发酵法生产漆酶来提高其生物降解性。探索了物理参数（温度，诱导剂浓度，底物负载）的影响，以使桑氏假丝酵母产生最大的漆酶产量。。结果表明，在30°C下使用无萃取OPEFB纤维，并添加4 mM 2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和10 g OPEFB负载可产生漆酶与原始OPEFB相比，它高2.1倍。通过傅立叶变换红外光谱（FTIR），热重分析（TGA）和扫描电子显微镜（SEM）分析报告了原料，化学品和漆酶处理的OPEFB的化学和表面结构的变化。模拟的技术经济分析表明，批量生产生物漆厂在工业规模饲料中以最大50吨OPEFB的速率生产漆酶具有经济可行性。根据模拟基准研究，由于资本（65.87％）和原材料成本（31.68％），漆酶生产的成本价格预测为14.26美元/千克。P. sanguineus不仅用于生物精炼行业，而且还用于生物制浆，生物漂白（在纺织和造纸行业）和降解酚类废物（染料，着色剂，农药废物）。