Abstract

Biological membranes include various lipids. A heterogeneity of lipid composition can lead to phase separation resulting in the formation of ordered lipid domains, which differ in lipid composition from the other (disordered) part of the membrane. Membrane deformations, which occur at the domain boundary, can affect the lateral distribution of various membrane inclusions. In this paper, in the framework of theory of elasticity of lipid membranes, the influence of the boundaries of lipid domains on the lateral distribution of amphipathic peptides adsorbed on the membrane is considered. Such peptides can cause the formation of through pores. We showed that with an increase in the concentration of amphipathic peptides on the membrane, they first line up near the boundary of the domain parallel to it, thus losing the ability to induce pore formation. Then, as the domain boundary is completely occupied, new peptides stand parallel to the line of peptides that are already at the boundary, at a distance of about 5 nm from the boundary. In this configuration, the probability of formation of through pores in the membrane is increased. Besides, it is shown that the spontaneous curvature of monolayers of the ordered domain and disordered membrane determines the energy of incorporation of peptides into the membrane and their distribution between the phases. However, the spontaneous curvature scarcely influences the interaction of amphipathic peptides with the boundary of the ordered domain.

中文翻译：

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有序的脂质域边界和两亲性肽的相互作用调节膜中孔形成的可能性。

摘要

生物膜包括各种脂质。脂质组成的异质性可导致相分离，导致形成有序的脂质结构域，该结构域的脂质组成与膜的其他（无序）部分不同。在畴边界处发生的膜变形会影响各种膜夹杂物的横向分布。本文在脂质膜弹性理论的框架内，考虑了脂质结构域边界对吸附在膜上的两亲性肽的侧向分布的影响。这样的肽可引起通孔的形成。我们发现，随着两亲性肽在膜上浓度的增加，它们首先会在与其平行的结构域边界附近排列，从而丧失诱导孔形成的能力。然后，当结构域边界被完全占据时，新肽与已经在边界处的肽线平行，离边界约5 nm。在这种构造中，增加了在膜中形成通孔的可能性。此外，显示出有序域和无序膜的单层的自发曲率决定了肽掺入膜中的能量及其在各相之间的分布。但是，自发曲率几乎不会影响两亲性肽与有序域边界的相互作用。膜中形成通孔的可能性增加。此外，显示出有序域和无序膜的单层的自发曲率决定了肽掺入膜中的能量及其在各相之间的分布。但是，自发曲率几乎不会影响两亲性肽与有序域边界的相互作用。膜中形成通孔的可能性增加。此外，显示出有序域和无序膜的单层的自发曲率决定了肽掺入膜中的能量及其在各相之间的分布。但是，自发曲率几乎不会影响两亲性肽与有序域边界的相互作用。