We studied the effects of lithium carbonate on the cell cycle, apoptosis, and autophagy in hepatocellular carcinoma-29 cells (HCC-29) in vitro. Flow cytofluorometry analysis revealed accumulation of G2/M-phase HCC-29 cells and increase in the number of apoptotic cells in 48 h after administration of 5 mM lithium carbonate. Induction of autophagy in HCC-29 cells was detected by transmission electron microscopy and immunofluorescence staining. Thus, lithium carbonate produces an antitumor effect by arresting cell cycle in the G2/M-phase and induction of apoptosis and autophagy in HCC-29 cells, which confirms the lithium potential as a promising drug for the treatment of hepatocellular carcinoma.

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碳酸锂对肝细胞癌 29 细胞周期、凋亡和自噬的体外影响

我们在体外研究了碳酸锂对肝细胞癌 29 细胞 (HCC-29) 细胞周期、细胞凋亡和自噬的影响。流式细胞荧光分析显示在施用 5 mM 碳酸锂后 48 小时内 G2/M 期 HCC-29 细胞的积累和凋亡细胞数量的增加。通过透射电子显微镜和免疫荧光染色检测 HCC-29 细胞中自噬的诱导。因此，碳酸锂通过在 G2/M 期阻滞细胞周期并诱导 HCC-29 细胞凋亡和自噬来产生抗肿瘤作用，这证实了锂作为治疗肝细胞癌的有前途的药物的潜力。