Investigating the effects of 405-nm, 532-nm, 650-nm, and 950-nm wavelengths of LLLTs (low-level laser therapies) on the orthodontic tooth movement in rats by using histological and immunohistochemical methods. Forty-five Wistar albino rats were randomly divided into 5 groups: control group (positive control: the left maxillary 1st molar side; negative control: the right maxillary 1st molar side), 405 nm LLLT group (Realpoo), 532 nm LLLT group (Realpoo), 650 nm LLLT group (Realpoo), and 940 nm LLLT group (Biolase). The left maxillary 1st molar teeth of all rats were applied mesially 50-g force. Starting from the 1st day, 48 h intervals, LLLT was applied in continuous wave mode and in contact with the tissue. The application area was approximately 1 cm2. The lasers were performed for 3 min on each surface (buccal, palatal, mesial), totally 9 min (total dose 54 J/cm2). The amount of the molar mesialization, the bone area between the roots, PDL (periodontal ligament) measurements, TRAP (tartrate-resistant acid phosphatase), and ALP (alkaline phosphatase) immunoreactivity intensity were calculated. The amount of the molar mesialization was significantly higher in the 650 nm LLLT group (mean 0.878 ± 0.201 mm; 95% CI (confidence interval) 0.724 and 1.032) than in the groups of positive control (mean 0.467 ± 0.357 mm; 95% CI 0.192 and 0.741) and 405 nm LLLT (mean 0.644 ± 0.261 mm; 95% CI 0.443 and 0.845) (p < 0.001). There were significant differences in the PDL-mesial (p = 0.042) and PDL-distal (p = 0.007) regions between the groups. The immunoreactivity intensity for TRAP-mesial was significantly higher in the positive control group (mean 109,420.33 ± 8769.17; 95% CI 100,217.65 and 118,623.02) than in the 405 nm (mean 91,678.83 ± 7313.39; 95% CI 84,003.9 and 99,353.77) and the 650 nm LLLT (mean 87,169.17 ± 4934.65; 95% CI 81,990.56 and 92,347.77) groups (p = 0.002). There was no statistically significant difference between the groups on immunoreactivity intensity with ALP staining. The results of this study show that LLLT with 650-nm wavelength increases orthodontic tooth movement more than 405-nm, 532-nm, and 940-nm LLLTs. The 940-nm and 650-nm LLLTs also increase the bone area between the roots by more than 405-nm and 532-nm wavelengths.

中文翻译：

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405、532、650和940 nm波长的低水平激光疗法对大鼠正畸牙齿运动的影响

使用组织学和免疫组织化学方法研究405 nm，532 nm，650 nm和950 nm波长的LLLT（低水平激光疗法）对大鼠正畸牙齿运动的影响。四十五只Wistar白化病大鼠随机分为5组：对照组（阳性对照组：左上颌第一磨牙侧;阴性对照组：右上颌第一磨牙侧），405 nm LLLT组（Realpoo），532 nm LLLT组（ Realpoo），650 nm LLLT组（Realpoo）和940 nm LLLT组（Biolase）。对所有大鼠的左上颌第一磨牙进行近50 g力的作用。从第一天开始，间隔48小时，以连续波模式并与组织接触应用LLLT。涂抹面积约为1平方厘米。在每个表面（颊，pa，内侧）进行激光3分钟，总共9分钟（总剂量54 J / cm2）。计算臼齿的齿化程度，牙根之间的骨面积，PDL（牙周膜）测量值，TRAP（抗酒石酸酸性磷酸酶）和ALP（碱性磷酸酶）的免疫反应强度。在650 nm LLLT组中，平均摩尔弥散化量（平均0.878±0.201 mm; 95％CI（置信区间）0.724和1.032）高于阳性对照组（平均0.467±0.357 mm; 95％CI） 0.192和0.741）和405 nm LLLT（平均0.644±0.261 mm; 95％CI 0.443和0.845）（p <0.001）。两组之间的PDL局部（p = 0.042）和PDL远端（p = 0.007）区域存在显着差异。阳性对照组的TRAP-小肠免疫反应强度显着更高（平均值109,420.33±8769.17; 95％CI 100,217。65和118,623.02）分别比405 nm（平均91,678.83±7313.39; 95％CI 84,003.9和99,353.77）和650 nm LLLT（平均87,169.17±4934.65; 95％CI 81,990.56和92,347.77）（p = 0.002）。ALP染色的免疫反应强度在两组之间没有统计学上的显着差异。这项研究的结果表明，波长为650 nm的LLLT可以使正畸牙齿的运动超过405 nm，532 nm和940 nm的LLLT。940 nm和650 nm的LLLT还会使根部之间的骨骼面积增加405 nm和532 nm以上的波长。ALP染色的免疫反应强度在两组之间没有统计学上的显着差异。这项研究的结果表明，波长为650 nm的LLLT可以使正畸牙齿的运动超过405 nm，532 nm和940 nm的LLLT。940 nm和650 nm的LLLT还会使根部之间的骨骼面积增加405 nm和532 nm以上的波长。ALP染色的免疫反应强度在两组之间没有统计学上的显着差异。这项研究的结果表明，波长为650 nm的LLLT可以使正畸牙齿的运动超过405 nm，532 nm和940 nm的LLLT。940 nm和650 nm的LLLT还会使根部之间的骨骼面积增加405 nm和532 nm以上的波长。