Polyphenols are allelochemicals secreted by aquatic plants that effectively control cyanobacteria blooms. In this study, sensitive response parameters (including CFPs) of Microcystis aeruginosa were explored under the stress of different polyphenols individually and their combination. The combined effects on M. aeruginosa were investigated based on the most sensitive parameter and cell densities. For pyrogallic acid (PA) and gallic acid (GA), the sensitivity order of parameters based on the EC₅₀ values (from 0.73 to 3.40 mg L^–1 for PA and from 1.05 to 2.68 mg L^–1 for GA) and the results of the hierarchical cluster analysis showed that non-photochemical quenching parameters [NPQ, q_N, q_N(rel) and q_CN] > photochemical quenching parameters [YII, q_P, q_P(rel) and q_L] or others [F_v/F_m, F’_v/F’_m, q_TQ and UQF_(rel)] > cell densities. CFPs were not sensitive to ellagic acid (EA) and (+)-catechin (CA). The sensitivity order of parameters for M. aeruginosa with PA-GA mixture was similar to that under PA and GA stress. The quantitative (Toxicity Index, TI) and qualitative (Isobologram representation) methods were employed to evaluate the combined effects of PA, GA, and CA on M. aeruginosa based on cell densities and NPQ. TI values based on the EC_{50 cells} suggested the additive effects of binary and multiple polyphenols, but synergistic and additive effects according to the EC_{50 NPQ} (varied from 0.16 to 1.94). In terms of NPQ of M. aeruginosa, the binary polyphenols exhibited synergistic effects when the proportion of high toxic polyphenols PA or GA was lower than 40%, and the three polyphenols showed a synergistic effect only at the ratio of 1:1:1. Similar results were also found by isobologram representation. The results showed that increasing the ratio of high toxic polyphenols would not enhance the allelopathic effects, and the property, proportion and concentrations of polyphenols played an important role in the combined effects. Compared with cell densities, NPQ was a more suitable parameter as evaluating indicators in the combined effects of polyphenols on M. aeruginosa. These results could provide a method to screen the allelochemicals of polyphenols inhibiting cyanobacteria and improve the inhibitory effects by different polyphenols combined modes.

多酚是水生植物分泌的能有效控制蓝藻水华的化感物质。在这项研究中，敏感的反应参数（包括CFP）铜绿微囊藻分别在不同多酚及其组合的胁迫下进行了探索。综合影响铜绿假单胞菌根据最敏感的参数和细胞密度进行了研究。对于焦没食子酸（PA）和没食子酸（GA），参数的灵敏度顺序基于EC ₅₀值（PA的0.73至3.40 mg L ^–1和GA的1.05至2.68 mg L ^–1）和结果聚类分析表明，非光化学猝灭参数[NPQ，q_ñ，q_N（rel）和q_CN ]>光化学猝灭参数[YII，q_P，q_P（rel）和q_L ]或其他[F_v /F_米，F'_v/F'_米，q_TQ和UQF _（rel） ]>单元密度。CFP对鞣花酸（EA）和（+）-儿茶素（CA）不敏感。参数的灵敏度顺序铜绿假单胞菌PA-GA混合物的应力与PA和GA应力下的应力相似。定量（毒性指数，TI）和定性（等效线图表示）方法用于评估PA，GA和CA对铜绿假单胞菌基于细胞密度和NPQ。基于EC _50细胞的TI值表明了二元和多种多酚的累加作用，但根据EC _{50 NPQ}（在0.16至1.94之间变化）产生了协同和累加作用。就NPQ而言铜绿假单胞菌，当高毒性多酚PA或GA的比例低于40％时，二元多酚具有协同作用，而三种多酚仅在1：1：1的比例下才具有协同作用。通过等效线图表示也发现了类似的结果。结果表明，增加高毒性多酚的比例不会增强化感作用，多酚的性质，比例和浓度在综合作用中起重要作用。与细胞密度相比，NPQ是更合适的参数，可作为多酚联合作用的评价指标。铜绿假单胞菌。这些结果可为筛选多酚抑制蓝细菌的化感物质和提高不同多酚结合方式的抑制效果提供一种方法。