In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-γ. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.

在这项研究中，首次对Ramson的抗增殖和抗氧化活性进行了综合分析，然后对其相关的微生物群和乳酸菌（LAB）的健康促进作用进行了分析。拉姆森（乌葱）由于其抗微生物和抗氧化活性，被认为是在传统医学中具有悠久历史的药用植物。在这项研究中体外 胃肠道消化对小鼠的细胞毒活性 乌si证明了针对人类恶性细胞系的提取物。硫代亚磺酸盐的降解产物，包括二烯丙基二硫化物在内的七种硫化合物被证明通过诱导G2 / M期的蓄积来抑制恶性细胞的增殖，并通过激活caspase-3和线粒体信号通路来诱导细胞凋亡。此外，乌si微生物，特别是具有潜在益生菌作用的乳酸菌，通过培养依赖法和培养依赖法[变性梯度凝胶电泳（DGGE）]进行了分析。获得的结果表明，最丰富的属是链球菌， 乳杆菌和 芽孢杆菌。的乳杆菌 属主要由 发酵乳杆菌。脉冲场凝胶电泳（PFGE）分析表明存在两种PFGE脉冲型。该菌株的益生菌潜力发酵乳杆菌 属于PFGE脉冲型1的BGSR163及其菌株 发酵乳杆菌对属于PFGE脉冲型2的BGSR227进行了表征。结果表明，这两种菌株对人类都是安全的，可以在模拟胃肠道条件下成功存活，具有暂时定居胃肠道（GIT）的潜力，并具有保护性免疫调节作用，诱导促炎性细胞因子IL17和调节性细胞因子IL10的产生，而减少促炎细胞因子IFN-γ的产生。总之，这项研究的结果表明，食用乌si 可能具有促进健康的特性，包括抗癌作用，而 发酵乳杆菌 从中分离出的菌株 乌si 叶子可以用作人类食用的益生菌。