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Plasmonic Assemblies for Real‐Time Single‐Molecule Biosensing
Small ( IF 13.0 ) Pub Date : 2020-12-01 , DOI: 10.1002/smll.202003934
Rachel E Armstrong 1 , Matěj Horáček 1 , Peter Zijlstra 1
Affiliation  

Their tunable optical properties and versatile surface functionalization have sparked applications of plasmonic assemblies in the fields of biosensing, nonlinear optics, and photonics. Particularly, in the field of biosensing, rapid advances have occurred in the use of plasmonic assemblies for real‐time single‐molecule sensing. Compared to individual particles, the use of assemblies as sensors provides stronger signals, more control over the optical properties, and access to a broader range of timescales. In the past years, they have been used to directly reveal single‐molecule interactions, mechanical properties, and conformational dynamics. This review summarizes the development of real‐time single‐molecule sensors built around plasmonic assemblies. First, a brief overview of their optical properties is given, and then recent applications are described. The current challenges in the field and suggestions to overcome those challenges are discussed in detail. Their stability, specificity, and sensitivity as sensors provide a complementary approach to other single‐molecule techniques like force spectroscopy and single‐molecule fluorescence. In future applications, the impact in real‐time sensing on ultralong timescales (hours) and ultrashort timescales (sub‐millisecond), time windows that are difficult to access using other techniques, is particularly foreseen.

中文翻译:

实时单分子生物传感的等离子组件

它们的可调光学特性和通用的表面功能化已激发了等离激元组件在生物传感,非线性光学和光子学领域的应用。特别是在生物传感领域,等离激元组件用于实时单分子传感的应用已取得了飞速发展。与单个粒子相比,将组件用作传感器可以提供更强的信号,对光学特性的更多控制以及更广泛的时间范围。在过去的几年中,它们被用来直接揭示单分子相互作用,机械性能和构象动力学。这篇综述总结了围绕等离激元组件构建的实时单分子传感器的发展。首先,简要概述它们的光学特性,然后描述了最近的应用。详细讨论了该领域当前的挑战以及克服这些挑战的建议。它们作为传感器的稳定性,特异性和敏感性为力谱和单分子荧光等其他单分子技术提供了一种补充方法。在未来的应用中,可以预见的是,实时感测对超长时标(小时)和超短时标(亚毫秒),以及使用其他技术难以访问的时间窗口的影响。
更新日期:2020-12-28
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