In this work, the delipidized and deproteinized bovine cancellous bone powder/poly–amino acid (DDBP/PAA) composite was fabricated by extrusion-injection molding method for the first time. After about 70% clearance rate by the delipidization and deproteinization procedures, the residual antigens of galactosyl α–(1, 3)–galactosyl β–1,4–N–aeetylglueosaminyl (α–Gal) and major histocompatibility complex (MHC) II were basically eliminated by the extrusion–injection molding process, which may cause high titer of antibody and lead to hyperacute rejection or chronic immune toxicity. Meanwhile, the natural BMP II and apatite in bovine bone were kept in DDBP/PAA composite. After 26 weeks of immersion in simulated body fluid, the DDBP/PAA composite remained the intact appearance, 96.4% of weight, and 69.2% of compressive strength, and these showed sufficient degradation stability. The composite also exhibited excellent attachment and proliferation abilities of mouse bone marrow mesenchymal stem cells (mMSCs). The results herein suggested that the DDBP/PAA composite was expected to be a load–bearing transplant with some natural ingredients for hard tissue repair.

中文翻译：

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体外制备具有低免疫原性、适当强度和细胞相容性的新型牛松质骨/聚氨基酸复合物

在这项工作中，首次采用挤出-注塑成型方法制备了脱脂脱蛋白牛松质骨粉/聚氨基酸（DDBP/PAA）复合材料。通过脱脂和去蛋白程序清除率约为 70% 后，半乳糖基 α-(1, 3)-半乳糖基 β-1,4-N-乙酰葡糖胺基 (α-Gal) 和主要组织相容性复合物 (MHC) II 的残留抗原分别为基本上通过挤出-注塑成型工艺消除，可能导致抗体滴度高，导致超急性排斥反应或慢性免疫毒性。同时，牛骨中的天然BMP II和磷灰石保留在DDBP/PAA复合物中。在模拟体液中浸泡 26 周后，DDBP/PAA 复合材料保持完整的外观，重量为 96.4%，抗压强度为 69.2%，并且这些显示出足够的降解稳定性。该复合材料还表现出优异的小鼠骨髓间充质干细胞 (mMSCs) 的附着和增殖能力。本文的结果表明，DDBP/PAA 复合材料有望成为一种具有一些用于硬组织修复的天然成分的承重移植物。