An Economical High‐Throughput “FP‐Tag” Assay for Screening Glycosyltransferase Inhibitors**,ChemBioChem

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An Economical High‐Throughput “FP‐Tag” Assay for Screening Glycosyltransferase Inhibitors**
ChemBioChem ( IF 2.6 ) Pub Date : 2020-12-01 , DOI: 10.1002/cbic.202000746
Xinjian Yin ₁ , Jiaxin Li ₁ , Senhua Chen ₁ , Yuping Wu ₁ , Zhigang She ₂ , Lan Liu _{1,

3} , Yue Wang ₄ , Zhizeng Gao _{1,

3}

Affiliation

High performance, low cost: A wash‐free, low‐cost fluorescent‐polarization‐based high‐throughput assay has been developed for O‐GlcNAc transferase. Such an assay is “catalytic”, thus only needing minimal amounts of enzymes and could potentially identify inhibitors targeting both the acceptor and donor binding‐sites.