Bovine alphaherpesvirus 2 (BoHV-2) - the agent of bovine herpetic mamillitis (BHM) – is related to Human alphaherpesviruses 1 and 2 (HHV-1, HHV-2) and, as such, has been proposed as a model for vaccine and drug testing. We herein investigated the anti-viral activity in vitro against BoHV-2 of three anti-herpetic drugs: Cidofovir (CDV), Fanciclovir (FAM), Foscarnet (PFA), and diphenyl disselenide (Ph₂Se₂)_, a compound that has showed activity against HHV-2. Plaque reduction assays (PRA) revealed a significant reduction in viral plaques (p < 0.05) in cells treated with Ph₂Se₂ (79.7% reduction) or CDV (62.8%). FAM treatment resulted in a slight decrease in plaque number (22.9%, p < 0.05); PFA showed no activity. The effects of Ph₂Se₂ and CDV, alone or in combination, were investigated in ewes inoculated with BoHV-2 transdermally and submitted to daily topic treatment. Virus inoculated ewes developed lesions progressing through the stages of hyperemia, large papules or depressed dark areas, followed by scab formation. Treatment with Ph₂Se₂ resulted in reduction in clinical score from day 10 pi onwards (P < 0.05), shortening of clinical course and reduction in duration of virus shedding (P < 0.05) compared to untreated controls. Combined treatment (Ph₂Se₂ + CDV) and CDV alone, also led to clinical improvement (P < 0.05), yet less pronounced and delayed. These results are promising towards the use of Ph₂Se₂, alone or in combination with anti-herpetic drugs, in the treatment of udder and teat lesions produced by BoHV-2 in dairy cows.

牛疱疹病毒2（BoHV-2）-牛疱疹性乳头炎（BHM）的病原-与人α疱疹病毒1和2（HHV-1，HHV-2）有关，因此，已被提议作为疫苗和流感病毒的模型药物测试。我们在本文中研究了三种抗疱疹药物对BoHV-2的体外抗病毒活性：西多福韦（CDV），泛昔洛韦（FAM），膦甲酸酯（PFA）和二苯基二硒代硒醚（Ph ₂ Se ₂）_，该化合物具有表现出针对HHV-2的活性。噬斑减少试验（PRA）显示， 用Ph ₂ Se ₂处理的细胞中的病毒斑块显着减少（p <0.05）（减少79.7％）或CDV（62.8％）。FAM治疗导致斑块数量略有下降（22.9％，p  <0.05）；PFA无活性。在透皮接种BoHV-2的母羊中研究了单独或组合使用Ph ₂ Se ₂和CDV的效果，并进行了每日主题治疗。接种了病毒的母羊会在充血，大丘疹或凹陷的深色区域发展成病变，随后形成结ab。用Ph ₂ Se ₂进行治疗可导致从pi开始第10天起临床评分降低（P  <0.05），缩短临床进程并减少病毒脱落持续时间（P <0.05）与未处理的对照组相比。联合治疗（Ph ₂ Se ₂  + CDV）和单独使用CDV，也导致临床改善（P <0.05），但不那么明显和延迟。这些结果有望将Ph ₂ Se ₂单独或与抗疱疹药物联合用于治疗BoHV-2在奶牛中引起的乳房和乳头病变。