Bacterial resistance has become a serious global health issue over the past decades due to the misuse and abuse of antibiotics. The development of effective antibacterial drugs with a new antibacterial mechanism is thus very critical. At present, there are many reports on the antibacterial properties and mechanisms of two-dimensional materials. Currently, the modification of g-C₃N₄ materials, as widely used two-dimensional materials, has become a key step in extending their potential applications in the field of antimicrobial therapy. In the present work, we prepared sulfur-doped g-C₃N₄ nanosheets (SCNNSs), which have good water dispersibility and sharp tips. The electrostatic interaction of SCNNSs with Tetrastigma hemsleyanum Diels & Gilg's polysaccharide-3 (THDG-3) provides a new strategy that can improve the killing efficiency of SCNNSs. In addition, THDG-3 can rapidly inhibit bacterial proliferation in the early stage of administration. Combined with the antibacterial activity of the SCNNSs, TPS/SCNNSs can inhibit bacteria for a long time. Scanning electron microscopy (SEM) observation of Escherichia coli (E. coli) after administration of the materials revealed that the bacterial cells were ruptured and their intracellular contents were completely separated from the cell membrane. Therefore, we speculate that the bactericidal mechanism of the TPS/SCNNSs probably involves cell membrane damage. In summary, TPS/SCNNSs achieve fast, long-term, dual-function bacteriostatic properties.

在过去的几十年中，由于滥用和滥用抗生素，细菌耐药性已成为严重的全球健康问题。因此，开发具有新抗菌机制的有效抗菌药物非常关键。目前，关于二维材料的抗菌性能和机理的报道很多。目前，作为广泛使用的二维材料，gC ₃ N ₄材料的改性已成为扩展其在抗菌治疗领域中潜在应用的关键步骤。在目前的工作中，我们制备了硫掺杂的gC ₃ N ₄纳米片（SCNNSs），它具有良好的水分散性和尖锐的尖端。SCNNSs与Tetrastigma hemsleyanum Diels＆Gilg的多糖3（THDG-3）提供了一种新的策略，可以提高SCNNS的杀灭效率。另外，THDG-3可以在给药的早期迅速抑制细菌增殖。结合SCNNS的抗菌活性，TPS / SCNNS可以长时间抑制细菌。的扫描电子显微镜（SEM）观察大肠杆菌（大肠杆菌）在施用材料后显示细菌细胞破裂并且其细胞内内容物与细胞膜完全分离。因此，我们推测TPS / SCNNSs的杀菌机制可能涉及细胞膜损伤。总之，TPS / SCNNS具有快速，长期的双重功能抑菌特性。