Abstract Pseudomonas spp. contamination was screened from a Minas Frescal cheese processing plant. Biofilm formation of Pseudomonas fluorescens and Pseudomonas aeruginosa and the effect of three sanitisers, peracetic acid, sodium hypochlorite, and chlorhexidine gluconate, on the biofilm production was also studied. The biofilms were formed in milk according to a central composite rotatable design (CCRD) at 0, 1.2, 4, 6.8, and 8 days and 7, 13, 27, 41, and 47 °C. Pseudomonas spp. counts were observed in milking machine, tank, wall, door handle, floor, drain, and cheese. All Pseudomonas spp. isolates showed lipolytic activity and some proteolytic activity. P. fluorescens was able to form biofilms at 9.8–47 °C and 0.9–8 days, while P. aeruginosa formed biofilms at 11–47 °C and 0.7–8 days. Complete biofilm removal was not observed for all sanitisers studied, showing difficulty in sanitising the surfaces after the biofilm formation.

中文翻译：

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模拟不锈钢表面上的荧光假单胞菌和铜绿假单胞菌生物膜的形成并通过消毒剂进行控制

摘要假单胞菌属。污染来自 Minas Frescal 奶酪加工厂。还研究了荧光假单胞菌和铜绿假单胞菌的生物膜形成以及三种消毒剂过乙酸、次氯酸钠和葡萄糖酸氯己定对生物膜产生的影响。根据中心复合可旋转设计 (CCRD)，在 0、1.2、4、6.8 和 8 天以及 7、13、27、41 和 47 °C 时在牛奶中形成生物膜。假单胞菌属 在挤奶机、水箱、墙壁、门把手、地板、排水管和奶酪中观察到计数。所有假单胞菌属。分离株表现出脂解活性和一些蛋白水解活性。P. fluorescens 能够在 9.8–47 °C 和 0.9–8 天形成生物膜，而 P. aeruginosa 在 11–47 °C 和 0.7–8 天形成生物膜。