Curcumin is antioxidative, liver protective and it has lipid regulatory effects. However, its in vivo efficiency is limited because of the low absorption rate and bioavailability. LVYP (Leu-Val-Tyr-Pro) is a tetrapeptide isolated, purified and identified from Corbicula fluminea with strong antioxidant activity. The interaction mechanisms between curcumin and LVYP were investigated using UV–vis absorption and fluorescence spectra. The results showed that the interaction between curcumin and LVYP was hydrophobic. When the temperature was increased from 25 to 37 °C, ΔG decreased and the number of ligand molecules increased, the interaction was quenched statically explained the formation of some compounds. On the other hand, dynamic quenching occurred when the temperature reached 60 °C, ΔG increased and the number of ligand molecules decreased, which indicated that temperature affected the interaction between curcumin and LVYP. This study also showed that LVYP could promote intestinal absorption of curcumin, predominantly in the ileum, which was explored using a non-everted intestinal sac model. In vivo experiments showed that the liver's protective effect with the combined administration of LVYP and curcumin was better than the curcumin alone, and LVYP could promote the hepatoprotective function of curcumin. It was hypothesized that the interaction of LVYP and curcumin enhanced the intestinal absorption rate of curcumin, thereby increasing its liver protection effect.

姜黄素具有抗氧化，保护肝脏和调节脂质的作用。然而，由于低吸收率和生物利用度，其体内效率受到限制。LVYP（LEU-VAL-酪氨酸-脯氨酸）是分离，纯化，并从识别的四肽河蚬具有很强的抗氧化活性。用紫外可见吸收和荧光光谱研究了姜黄素和LVYP之间的相互作用机理。结果表明姜黄素和LVYP之间的相互作用是疏水的。当温度从25升高到37°C时，ΔG降低，配体分子数增加，相互作用被静态淬灭，解释了某些化合物的形成。另一方面，当温度达到60°C时发生动态猝灭，ΔG增加，配体分子数量减少，这表明温度影响姜黄素与LVYP之间的相互作用。这项研究还表明，LVYP可以促进姜黄素的肠吸收，主要是在回肠中，这是使用非衰减肠囊模型进行研究的。体内实验表明，LVYP和姜黄素联合给药对肝脏的保护作用优于单独的姜黄素，LVYP可以促进姜黄素的肝保护作用。据推测，LVYP与姜黄素的相互作用增强了姜黄素的肠吸收率，从而增强了其肝脏保护作用。