Present inquisition was undertaken to evaluate the genotoxicity of naphthalene-2-sulfonate (2NS), a sulfonated aromatic compound and a momentous intermediate involved in the synthesis of dyes and surfactants, in fresh water fish, Channa punctatus. After LC₅₀ determination, two sublethal concentrations i.e. 2.38g/15g b.w. (1/4 of LC₅₀) and 4.77 g/15g b.w. (1/2 of LC₅₀) were selected for studying acute exposure. For evaluating sub chronic exposure 1/10th (0.238g/L) and 1/20th (0.119 g/L) of safe application rate (SAR) were reckoned. Blood samples were collected after 24, 48, 72, and 96 hours exposure period to study acute effect, and after 30 and 60 days exposure period for sub-chronic effect. Symbolic elevation in time and dose dependent DNA damage was observed by comet assay as well as micronucleus test revealing maximum damage after 60 days of exposure. After cessation of exposure to 2NS, evident recovery was observed after 30 days. Along with comet assay and micronucleus test, spectroscopic evaluation of DNA damage was also noted using Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR). The biomolecular range (800cm^-1 - 1800cm-¹) in lyophilized red blood cell’s extracted from 60 days exposed as well as control group exhibit significant alterations in their nucleic acid indicated through multivariate analysis i.e. Principal Component Analysis (PCA). Further structural analysis of erythrocytes in maximally damaged group using Scanning Electron Microscopy was performed. Thus the study proposed the genotoxic impact of 2NS which is further supported by other toxicity markers like ATR-FTIR and Scanning Electron Microscopy.

目前的调查是在淡水鱼类Channa punctatus中评估萘-2-磺酸盐（2NS），磺化芳族化合物和参与染料和表面活性剂合成的重要中间体的遗传毒性。LC ₅₀测定后，两个亚致死浓度，即2.38g / 15g bw（LC _50的1/4 ）和4.77g / 15g bw（LC _50的1/2））用于研究急性暴露。为了评估亚慢性暴露，安全施用率（SAR）分别为1/10（0.238g / L）和1/20（0.119 g / L）。在暴露24、48、72和96小时后收集血液样本以研究急性效应，在暴露30和60天后以亚慢性效应收集血样。通过彗星试验以及微核试验观察到时间和剂量依赖性DNA损伤的象征性升高时间，显示暴露60天后最大损伤。停止接触2NS后，在30天后观察到明显的恢复。除了彗星分析和微核试验，还使用衰减全反射傅立叶变换红外（ATR-FTIR）技术对DNA损伤进行了光谱评估。生物分子范围（800厘米^-1 - 1800cm- ¹暴露于60天的冻干红细胞提取物中以及对照组中，通过多变量分析（即主成分分析（PCA））表明其核酸发生了显着变化。使用扫描电子显微镜对最大受损组的红细胞进行进一步的结构分析。因此，该研究提出了2NS的遗传毒性影响，其他毒性标记（例如ATR-FTIR和扫描电子显微镜）进一步证明了2NS的遗传毒性。