Abstract This work synthesized the CeO2 NPs using the plant extract of Stachys japonica Miq and determined their antioxidant and antidiabetic activities. The characterization results demonstrated the successful formation of spherical shaped CeO2 NPs with average size of 21 nm with PDI of 0.121. The FTIR peak of CeO2 NPs revealed the capping of phyto-molecules and a peak at 470.92 indicated the formation of Ce-O of CeO2 NPs. The CeO2 NPs scavenged the free radicals of ABTS and DPPH, with IC50 of 12.16± 0.12 μg. mL-1 and 109.5 ± 0.26 μg. mL-1 respectively. The 50 μg.mL-1 of CeO2 NPs showed the maximum inhibitory effect on α-glucosidase (19.78±0.95%) and α-amylase (43.24±1.14%). The cellular assay revealed the non-toxicity of CeO2 NPs in NIH3T3 and HepG2 cells. Cell antioxidant assay demonstrated that CeO2 NPs attenuated the STS induced-oxidative stress in NIH3T3 cells through preventing the MMP loss and nucleus damage. The treatment of CeO2 NPs was broken the insulin resistance in IR-HepG2 cells and increased the glucose uptake through strengthening the mitochondria from oxidative stress. These antioxidant and antidiabetics properties of CeO2 NPs have opened a new avenue for extending future study on CeO2 NPs regulated glucose metabolism and its underlying molecular mechanism for beneficial therapy of diabetes.

中文翻译：

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小鼠成纤维细胞 NIH3T3 和胰岛素抵抗 HepG2 细胞中生物相容性氧化铈 (CeO2) 纳米颗粒的抗氧化和抗糖尿病特性

摘要 本工作利用日本水苏的植物提取物合成了 CeO2 NPs，并测定了它们的抗氧化和抗糖尿病活性。表征结果表明成功形成了平均尺寸为 21 nm、PDI 为 0.121 的球形 CeO2 NP。CeO2 NPs 的 FTIR 峰揭示了植物分子的封端，470.92 处的峰表明 CeO2 NPs 形成了 Ce-O。CeO2 NPs清除ABTS和DPPH的自由基，IC50为12.16±0.12 μg。mL-1 和 109.5 ± 0.26 μg。分别为 mL-1。50 μg.mL-1 的 CeO2 NPs 对 α-葡萄糖苷酶 (19.78±0.95%) 和 α-淀粉酶 (43.24±1.14%) 的抑制作用最大。细胞分析表明，CeO2 NPs 在 NIH3T3 和 HepG2 细胞中无毒性。细胞抗氧化试验表明，CeO2 NPs 通过防止 MMP 丢失和细胞核损伤来减弱 NIH3T3 细胞中 STS 诱导的氧化应激。CeO2 NPs 的处理打破了 IR-HepG2 细胞的胰岛素抵抗，并通过加强线粒体免受氧化应激来增加葡萄糖摄取。CeO2 NPs 的这些抗氧化和抗糖尿病特性为扩展未来对 CeO2 NPs 调节葡萄糖代谢及其有益治疗糖尿病的潜在分子机制的研究开辟了新的途径。