Abstract

Ajuga turkestanica (Rgl.) Briq. (Lamiaceae) callus cultures were obtained from a wild plant, and the morphogenesis processes in these cultures were studied. It was possible to obtain gemmogenesis or rhizogenesis in the first six to eight growth cycles, depending on the hormonal composition of the medium; the capacity for morphogenesis was lost after 10–15 growth cycles. Suspension cell cultures were initiated from some calluses with strong growth. As a result, about 100 calluses and suspension plant cell lines of A. turkestanica were obtained; a number of them were characterized in terms of growth rate and were analyzed via HPLC-MS for the presence of phytoecdysteroids. 20-Hydroxyecdysone and turkesterone were found in most of the studied lines; the content of the first compound in the most productive lines was 30–50 times higher than in the second (2.0–2.5 mg/g versus 0.04– 0.05 mg/g dry weight, respectively). An increase in the phytoecdysteroid content in plant cells cultured in vitro was observed by the end of the growing cycle. However, phytoecdysteroids were not found in many of the obtained plant cell lines. Further research is needed to clarify the causes of the presence or absence of phytoecdysteroids in A. turkestanica plant cell cultures.

中文翻译：

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产生植物蜕皮类固醇的土耳其假丝酵母植物细胞培养的获得和特性

摘要

Ajuga turkestanica（ Rgl。）（唇形科）愈伤组织培养物是从野生植物中获得的，并且研​​究了这些培养物中的形态发生过程。根据培养基的激素成分，在最初的六到八个生长周期中可能获得成胚或发根。10–15个生长周期后，失去了形态发生能力。悬浮细胞培养是从一些生长强劲的老茧开始的。结果，约有100个土耳其土生曲霉的愈伤组织和悬浮植物细胞系获得; 其中许多通过生长速率进行了表征，并通过HPLC-MS分析了植物蜕皮激素的存在。在大多数研究品系中都发现了20-羟基蜕皮酮和turkesterone。生产效率最高的产品中第一种化合物的含量比第二种产品高30-50倍（分别为2.0-2.5 mg / g和0.04-0.05 mg / g干重）。在生长周期结束时，观察到了体外培养的植物细胞中植物蜕皮激素含量的增加。但是，在许多获得的植物细胞系中未发现植物蜕皮激素。需要进一步的研究来阐明土库曼斯坦A. turkestanica植物细胞培养物中是否存在植物蜕皮激素的原因。