The efficiency of the bioaugmentation of nitrifying bacteria into biofilm microbiocenosis with 30 days of continuous biofiltration of a solution of municipal model wastewater has been assessed. The laboratory setup consisted of two parallel operating biofilters. Cultures of ammonium-oxidizing and nitrite-oxidizing bacteria of the Nitrobacter genus were sequentially introduced into one of them after the initial period. It was established that the bioaugmentation of ammonium-oxidizing bacteria into the biofilm microbiocenosis led to an increase in the efficiency of the removal of ammonium nitrogen by an average of 1.6 times as compared to the control biofilter. The subsequent bioaugmentation of nitrite-oxidizing bacteria caused an increase in the amount of nitrates in purified water by an average of two times. The bioaugmentation of nitrifying bacteria in the biofilm microbiocenosis intensified the nitrification process. The quantitative and qualitative identification of microorganisms via fluorescence in situ hybridization showed an increased number of nitrifying microorganisms in the biofilm of the experimental biofilter and a correlation between this characteristic and the biotransformation of nitrogen compounds, which confirms the efficiency of the introduction of microorganisms into the biofilm.

已经评估了市政模型废水溶液连续生物过滤30天后硝化细菌向生物膜微生物集聚中的生物强化效率。实验室设置由两个平行运行的生物滤池组成。硝化细菌的氧化铵和亚硝酸盐氧化细菌的培养初始阶段后，将属依次引入其中一个。已经确定，与对照生物滤池相比，铵氧化细菌向生物膜微生物群落的生物增强导致去除铵态氮的效率平均提高了1.6倍。随后亚硝酸盐氧化细菌的生物增强作用使纯净水中的硝酸盐量平均增加了两倍。在生物膜微生物群落中，硝化细菌的生物增强作用增强了硝化过程。通过荧光原位荧光定量和定性鉴定微生物 杂交显示实验性生物滤池生物膜中硝化微生物的数量增加，并且该特征与氮化合物的生物转化之间具有相关性，这证实了将微生物引入生物膜的效率。