Several serum-free media have been developed and are successfully used at present for the cultivation of various recombinant eukaryotic cell lines. Therapeutic recombinant IgA antibodies have recently been increasingly used along with numerous IgG1 isotype antibodies. Therefore, it became necessary to improve the growth characteristics, metabolism, and productivity of cell cultures producing these antibodies. The stimulating effect of a complex supplement containing Zn salts and FGF-2 on cell cultivation on basal media, DMEM and IMDM, was revealed in this work. The addition of dextran sulfate sodium salt and iron citrate to the basal medium was accompanied by improved productivity of stable IgG- and IgA-producing cell lines, as well as the homogeneity and density of cell cultures, which is most important in IgA-antibody production.

已经开发了几种无血清培养基，目前已成功用于多种重组真核细胞系的培养。最近，治疗性重组IgA抗体与众多IgG1同种型抗体越来越多地被使用。因此，有必要提高产生这些抗体的细胞培养物的生长特性，代谢和生产率。这项工作揭示了含锌盐和FGF-2的复合补品对基础培养基DMEM和IMDM上细胞培养的刺激作用。向基础培养基中添加硫酸葡聚糖硫酸钠盐和柠檬酸铁可提高稳定的产生IgG和IgA的细胞系的生产力，以及细胞培养物的均质性和密度，这在生产IgA抗体中最重要。