Leishmaniasis is a group of infectious and non-contagious severe parasitic diseases, caused by protozoans of the Leishmania genus. Natural products characterize a rich source of prospective chemical entities for the development of new effective drugs for neglected diseases. Scientific evaluation of medicinal plants has made it possible to use some metabolites from flavonoids and polyphenols compounds for the treatment of parasitic diseases. Therefore, we aimed in this study to evaluate the protective effect of Silver nanoparticles (Ag-NPs) biosynthesized using Fig and Olive extracts (NFO) against Cutaneous leishmaniasis in female Balb/c mice. A total of 70 mice were used and divided into seven groups. Treatment was initiated when local lesions were apparent, we found Fig and Olive extracts were found to be a good source for the synthesis of (Ag-NPs), their formation was confirmed by color change and stability in solution. Nanoparticles biosynthesized using Fig and Olive extracts induced a reduction in the average size of cutaneous leishmaniasis lesions compared with the untreated mice. Moreover, nanoparticles treatment decreased oxidative stress (LPO, NO), down regulation gene expression levels (TNF-α, IL-1β and BAX) and this antileishmanial activity of nanoparticles was associated with enhanced antioxidant enzyme activities. In addition, histopathological evaluation proved the antileishmanial activity of nanoparticles compared to the positive control. Therefore, we aimed in this study to evaluate the protective effect of silver nanoparticles biosynthesized using Fig and Olive extracts against cutaneous lesions induced by Leishmania major infection through their anti-inflammatory, antioxidant activities and faster clinical efficacy than standard pentavalent antimonial treatment.

中文翻译：

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使用无花果和橄榄提取物生物合成的银纳米颗粒对雌性 Balb/c 小鼠皮肤利什曼病引起的炎症的影响。


利什曼病是由利什曼原虫属原生动物引起的一组传染性和非传染性严重寄生虫病。天然产物具有丰富的前景化学实体来源，可用于开发治疗被忽视疾病的新有效药物。对药用植物的科学评估使得利用类黄酮和多酚化合物的一些代谢物治疗寄生虫病成为可能。因此，我们的研究目的是评估使用无花果和橄榄提取物 (NFO) 生物合成的银纳米颗粒 (Ag-NP) 对雌性 Balb/c 小鼠皮肤利什曼病的保护作用。总共使用70只小鼠并分为七组。当局部病变明显时开始治疗，我们发现无花果和橄榄提取物是合成（Ag-NP）的良好来源，其形成通过颜色变化和溶液稳定性得到证实。与未经治疗的小鼠相比，使用无花果和橄榄提取物生物合成的纳米颗粒可减少皮肤利什曼病病变的平均大小。此外，纳米颗粒治疗可降低氧化应激（LPO、NO）、下调基因表达水平（TNF-α、IL-1β 和 BAX），并且纳米颗粒的这种抗利什曼活性与增强的抗氧化酶活性相关。此外，组织病理学评估证明了与阳性对照相比纳米颗粒的抗利什曼活性。 因此，我们在本研究中旨在评估使用无花果和橄榄提取物生物合成的银纳米颗粒对利什曼原虫主要感染引起的皮肤损伤的保护作用，其抗炎、抗氧化活性以及比标准五价锑治疗更快的临床疗效。