Environmental sequencing surveys unveil an unexpected magnitude of protist biodiversity and help to understand environmental community structure as well as biogeographical patterns. The interpretation of these data is still hindered by the lack of a verified and reliable reference database, which is the important basis for all analyses. References should rely on detailed and valid taxonomical descriptions including both morphology and autecological properties. In fact, obtaining such data is still a major challenge as cultivation-based approaches are very selective. In the present study, we highlight the potential to resample habitats which showed phylogenetically interesting sequences from environmental molecular surveys. We have been able to reveal a choanoflagellate species with the use of a single cell isolation approach in order to achieve a morphological description to the target sequence. This new species, Enibas thessalia sp. nov. now extends a recently described monospecific genus. In addition, we illustrate a nudiform lorica reproduction of the genus Enibas by observation of living cells. The genus belongs to the family of Acanthoecidae, which comprises five genera. The morphology of the genus Enibas shows a striking resemblance to the genus Stephanoeca, which belongs to the other family of loricate choanoflagellates, the Stephanoecidae, indicating that morphology alone might not reflect phylogenetic relations. We demonstrate that mapping sequences to a taxonomical description of species is a valuable tool to verify the organism behind an environmental amplicon. We emphasize the urgent need of integrative taxonomy matching molecular data with morphological features to verify the outcome of phylogenetic analyses.

环境排序调查揭示了原始生物多样性的惊人数量，并有助于了解环境社区的结构以及生物地理模式。这些数据的解释仍然由于缺乏经过验证的可靠参考数据库而受到阻碍，这是所有分析的重要基础。参考文献应依赖详细有效的分类学描述，包括形态学和自律学性质。实际上，由于基于耕种的方法具有很高的选择性，因此获取此类数据仍然是一项重大挑战。在本研究中，我们强调了对重新采样生境的潜力，这些生境显示了来自环境分子调查的系统发育有趣序列。我们已经能够使用单细胞分离方法来揭示鞭毛藻物种，以实现对靶序列的形态学描述。这个新物种Enibas色萨利SP。十一月 现在扩展最近描述的单特异性属。另外，我们通过观察活细胞说明了埃尼巴斯属的裸子状lorica繁殖。该属属于棘皮动物科，由五个属组成。Enibas属的形态与Stephanoeca属极为相似属于鞭毛鞭毛科的另一个家族，Stephanoecidae，表明仅形态学可能无法反映系统发育关系。我们证明了将序列映射到物种的分类学描述是验证环境扩增子背后生物的宝贵工具。我们强调迫切需要将分类数据与分子特征相匹配的综合分类法，以验证系统发育分析的结果。