The effects of aeration regimes (intermittent and continuous aeration) on nitritation performance and biofilm EPS composition were evaluated in moving bed biofilm reactors (MBBRs), and a hypothesis that the aeration regimes affect EPS composition by affecting the microbial activity and sludge discharge content was proposed. The effluent NO₂⁻/NH₄⁺ ratio corresponded to that of an anammox reaction (1.07 ± 0.20) for the MBBR with continuous aeration (MBBR_con.), while that in the MBBR with intermittent aeration (20 min on/15 min off) (MBBR_int.) was relatively lower (0.75 ± 0.19). Furthermore, the activity of ammonia-oxidizing bacteria in MBBR_con. was 0.4–7.9 mg-N·L⁻¹·h⁻¹ more than that in MBBR_int., which was consistent with the lower proportion of dead cells in MBBR_con. compared with MBBR_int. (9.4% vs. 31.8%). The higher microbial activity in MBBR_con. led to more sludge discharge than MBBR_int., which was reflected in the higher biofilm detachment rate in MBBR_con. compared with MBBR_int. (0.15 ± 0.02 vs. 0.11 ± 0.02 g m⁻²·d⁻¹). The ratio of humic substances to polysaccharides in the EPS was high (0.96 ± 0.08) in the detachment biomass, while the ratios in the nitritation biofilm on carriers from MBBR_con. and MBBR_int. were 0.52 ± 0.13 and 0.72 ± 0.16, respectively. We hypothesized that biofilm matrix with high ratios of humic substances to polysaccharides are structurally unstable and prone to fall off. In addition, the higher proportion of dead cells in MBBR_int. made the proportion of humic substances in EPS high. Meanwhile, less sludge discharge in MBBR_int. than MBBR_con. caused more humic substances to accumulate in the biofilm. These was responsible for the higher ratio of humic substances to polysaccharides in MBBR_int. compared with MBBR_con. The findings elucidate the connection between aeration regimes and biofilm EPS composition, and guide the choice of aeration regimes in the design of biofilm reactors for partial nitritation.

在移动床生物膜反应器（MBBR）中评估了曝气方式（间歇和连续曝气）对硝化性能和生物膜EPS组成的影响，并提出了一种假设，即曝气方式通过影响微生物活性和污泥排放量来影响EPS组成。 。的流出物NO ₂ ^- / NH ₄ ⁺之比对应于该一个厌氧氨氧化反应（1.07±0.20），用于与连续曝气的MBBR（MBBR的_CON。 ），而在与间歇曝气的MBBR（20分钟开/ 15分钟关闭）（MBBR _int。）相对较低（0.75±0.19）。此外，MBBR中氨氧化细菌的活性也有所提高_。为0.4–7.9 mg-N·L ^-1·h ^-1比MBBR _int高。，这与MBBR _con中较低的死细胞比例一致_。与MBBR _int相比_。（9.4％比31.8％）。MBBR _con中较高的微生物活性_。导致污泥排放量超过MBBR _int。，这反映在MBBR _con较高的生物膜分离率_。与MBBR _int相比_。（0.15±0.02vs.0.11±0.02g m ^-2 ·d ^-1）。在分离生物质中，EPS中腐殖质与多糖的比率较高（0.96±0.08），而MBBR载体上的硝化生物膜比率较高_。和MBBR_诠释 分别为0.52±0.13和0.72±0.16。我们假设腐殖质与多糖比例高的生物膜基质在结构上不稳定，容易脱落。此外，MBBR _int中死细胞的比例更高_。使腐殖质在EPS中的比例很高。同时，MBBR _int的污泥排放量更少_。比MBBR _con。导致更多的腐殖质积聚在生物膜中。这些是造成MBBR _int中腐殖质与多糖比例更高的原因_。与MBBR _con相比。这些发现阐明了曝气方式与生物膜EPS组成之间的联系，并指导了用于部分硝化的生物膜反应器设计中的曝气方式的选择。