Di-butyl phthalate (DBP) is an extensively applied synthetic plasticizer, toxic organic compound with elevated concentrations in aquatic and terrestrial ecosystem that cause serious risk to the human health. A marine bacterium Rhodovulum sp. DBP07 isolated from sea water with proficient of efficiently degrading DBP. The maximum DBP degradation (70.2%) and the cell growth (1.3 OD_600nm) were observed at 600 mg/L. The DBP degradation characteristics of the isolate Rhodovulum sp. DBP07 with diverse preliminary concentrations of DBP was found to be 200 ˃ 400 ˃ 600 ˂ 800 ˂ 1000 mg/L DBP. Glucose was identified as most favorable nutrient factor for the enhanced growth and showed 79.8 and 77.4% of degradation rate at 5.0 and 2.0 g/L respectively. The influence of the carbon sources on DBP degradation was found to be Glucose ˃ fructose ˃ sucrose ˃ maltose ˃ lactose ˃ citric acid ˃ starch. Box-Behnken (BBD) statistical optimization results showed enhanced DBP biodegradation rate (91.1%) at pH 7.0, 3% of NaCl concentration with 3 days of incubation. Two intermediate compounds were observed in the retention times of 10.8 and 12.2 which are identified as diethyl phthalate (DEP) and mono-nbutyl phthalate (MBP) using Gas chromatography mass spectroscopy (GC-MS). Furthermore, the phthalate (pht) gene expression pattern under DBP stress was analyzed using RT-qPCR and the maximum fold change (5.7 fold) was observed at 3 day of incubation. Overall, the observed results indicate the possibility of utilizing Rhodovulum sp. for remediation of DBP contaminated environment.

邻苯二甲酸二丁酯（DBP）是一种广泛应用的合成增塑剂，是一种有毒的有机化合物，在水生和陆地生态系统中浓度较高，会对人体健康造成严重威胁。海洋细菌Rhodovulum sp.。从海水中分离出的DBP07具有有效降解DBP的能力。在600 mg / L时观察到最大的DBP降解（70.2％）和细胞生长（1.3 OD _600nm）。红景天分离物的DBP降解特性sp。初步浓度为DBP的DBP07为200×400×600×800×1000 mg / L DBP。葡萄糖被认为是促进生长的最有利营养因子，在5.0 g / L和2.0 g / L时分别显示出79.8％和77.4％的降解率。发现碳源对DBP降解的影响是葡萄糖˃果糖˃蔗糖˃麦芽糖˃乳糖˃柠檬酸˃淀粉。Box-Behnken（BBD）统计优化结果显示，培养3天后，pH 7.0，NaCl浓度为3％时，DBP的生物降解率提高了（91.1％）。使用气相色谱质谱（GC-MS），在保留时间10.8和12.2中观察到两种中间体化合物，分别为邻苯二甲酸二乙酯（DEP）和邻苯二甲酸单正丁酯（MBP）。此外，使用RT-qPCR分析DBP胁迫下邻苯二甲酸酯（pht）基因的表达模式，并在孵育3天时观察到最大倍数变化（5.7倍）。总体而言，观察到的结果表明了利用红景天菌 修复受DBP污染的环境。