Salmon calcitonin (sCT) peptide has been widely used in the treatment of osteoporosis, Paget's disease and chronic pain. In this paper, we first tried to determine the best suitable force field based on the stability and helix properties of wild type sCT and we found that CHARMM27 is the most suitable one among the four different popular force fields tested. We secondly performed triple MD simulations of 300 ns at 310 K for each sCT mutant system. The simulation results indicated that the substitution of the Gln14 with Glu enable to formation of many additional salt bridges increasing stability. It was also observed that the substitution of the Gly10 with the Lys has enhanced helix-tail interaction. Consequently, we expect that our detailed simulation results can shed light on the future theoretical and experimental mutational studies of the sCT and help the development for its therapeutic purposes.

鲑鱼降钙素（sCT）肽已广泛用于治疗骨质疏松症，佩吉特氏病和慢性疼痛。在本文中，我们首先尝试根据野生型sCT的稳定性和螺旋特性确定最合适的力场，并发现CHARMM27是所测试的四个不同的流行力场中最合适的一个。其次，我们对每个sCT突变体系统在310 K处进行了300 ns的三倍MD模拟。模拟结果表明，用Glu取代Gln14可以形成许多增加稳定性的盐桥。还观察到用Lys取代Gly10具有增强的螺旋尾相互作用。所以，