Abstract A method was developed for rapid qualitative determination of lignocellulose in the tobacco cell wall by utilizing 2D heteronuclear single quantum coherence NMR spectra (2D HSQC NMR). Traditional methods for analyzing the structure of lignocellulose involve many steps of separation and extraction, which is labor-intensive. In this work, the whole cell wall was milled and dissolved in deuterium solvent. The solvent dimethylsulfoxide (DMSO-d6) containing hexamethylphosphoramide (HMPA-d18) enhanced swelling of the sample and gave high-resolution spectra. The tobacco samples are ball milled at different ball milling times, and the state of the particles is observed through an electron microscope, and then the probability of the particles being less than 5 µm is counted. Through the comparison of the abundance and integration of the peak signals in the spectra under different transmittances, it was determined that when the milling time was 6 h, the quality of the NMR spectra was the best. The optimum conditions of characterizing tobacco structure were DMSO-d6/HMPA-d18 solution and 6 h milling time. Under these conditions, complete representation of the structure of lignocellulose and simplified process could be achieved. Graphical abstract

中文翻译：

---

用优化程序通过溶液态二维核磁共振光谱阐明植物细胞壁的结构特征

摘要 建立了一种利用二维异核单量子相干核磁共振谱（2D HSQC NMR）快速定性测定烟草细胞壁中木质纤维素的方法。传统的木质纤维素结构分析方法涉及许多分离和提取步骤，劳动强度大。在这项工作中，整个细胞壁被研磨并溶解在氘溶剂中。含有六甲基磷酰胺 (HMPA-d18) 的溶剂二甲基亚砜 (DMSO-d6) 增强了样品的溶胀性并提供了高分辨率光谱。将烟草样品在不同球磨时间进行球磨，通过电子显微镜观察颗粒状态，然后计算颗粒小于5μm的概率。通过比较不同透射率下光谱中峰信号的丰度和积分情况，确定球磨时间为6 h时，NMR光谱的质量最好。表征烟草结构的最佳条件是DMSO-d6/HMPA-d18溶液和6 h研磨时间。在这些条件下，可以实现木质纤维素结构的完整表示和简化的过程。图形概要 可以实现木质纤维素结构的完整表示和简化的过程。图形概要 可以实现木质纤维素结构的完整表示和简化的过程。图形概要