The basic helix-loop-helix (bHLH) protein transcription factor family is the most widely distributed transcription factor family in eukaryotes. Members of this family play important roles in secondary metabolic biosynthesis, signal transduction, and plant resistance. Research on the bHLH family in animals is more extensive than that in plants, and members of the family in plants are classified according to the classification criteria for those in animals. To date, no research on the bHLH gene family in Pinus massoniana (Masson pine) has been reported. In this study, we identified 88 bHLH genes from four transcriptomes of Masson pine and performed bioinformatics analysis. These genes were divided into 10 groups in total. RT-PCR analysis revealed that the expression levels of the six genes increased under abiotic stress and hormone treatments. These findings will facilitate further studies on the functions of bHLH transcription factors.

中文翻译：

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马尾松基本螺旋-环状-螺旋转录因子家族的鉴定与鉴定

基本的螺旋-环-螺旋（bHLH）蛋白转录因子家族是真核生物中分布最广泛的转录因子家族。该家族的成员在次级代谢生物合成，信号转导和植物抗性中起重要作用。对动物中bHLH家族的研究比对植物的广泛，并且根据动物中bHLH家族的分类标准对植物中的bHLH家族进行了分类。迄今为止，尚未报道对马尾松（Masson pine）中bHLH基因家族的研究。在这项研究中，我们确定了88 bHLH来自马尾松四个转录组的基因，并进行了生物信息学分析。这些基因共分为10组。RT-PCR分析显示，在非生物胁迫和激素处理下，这六个基因的表达水平增加。这些发现将有助于进一步研究bHLH转录因子的功能。