Five new chlorinated azaphilone derivatives, tersaphilones A-E along with three known analogues were isolated from the deep-sea-derived fungus Phomopsis tersa FS441. Structures of the new compounds with absolute configurations were determined by comprehensive chemical analyses including spectroscopic techniques, ECD calculation, and single crystal X-ray diffraction. Among them, tersaphilone B possessed the unique 6/6-6 carbon skeleton with a cleaved tetrahydrofuranyl ring. Besides, tersaphilones D and E were a pair of diastereomers featuring a characteristic epoxide ring, which were rarely-occurring in azaphilones. Moreover, all compounds were evaluated for their anticancer activity using a panel of four tumor cell lines, wherein the novel tersaphilones D and E showed significant cytotoxicity against MCF-7, SF-268, and A549 cell lines with the IC₅₀ values ranging from 5.4 to 8.3 μM.

从深海真菌Phomopsis tersa中分离出五个新的氯化氮杂苯甲酮衍生物，tersaphilones AE和三个已知类似物FS441。通过全面的化学分析，包括光谱技术，ECD计算和单晶X射线衍射，可以确定具有绝对构型的新化合物的结构。其中，地塞米松B具有独特的6 / 6-6碳骨架，带有裂解的四氢呋喃基环。此外，苯三氮酮D和E是一对具有特征性环氧环的非对映异构体，在氮杂苯酮中很少出现。此外，使用一组四个肿瘤细胞系对所有化合物的抗癌活性进行了评估，其中新的萜品类化合物D和E对MCF-7，SF-268和A549细胞系显示出显着的细胞毒性，IC ₅₀值为5.4至 8.3μM .