P. aeruginosa is of particular importance due to its numerous pathogens and the spread of its multidrug-resistant strains around the world. Hence there is a need to develop an effective vaccine to prevent the diseases with P. aeruginosa. The aim of present study was to evaluate the immunogenicity of alginate (Alg) antigen in conjugation with SLN as a candidate for nanovaccine against P. aeruginosa in mouse model. Alginate is a weak immunogen, but the immune responses produced by alginate are effective in killing Pseudomonas bacteria. To increase the immunogenicity of alginate, SLN was used that is useful in drug delivery and can boost prolonged effectiveness. The results of ELISA and opsonophagocytosis tests showed that Alg-SLN conjugate has a better ability to stimulate the immune system to produce more immunoglobulins with better performance compared to alginate antigen alone. The challenge test also demonstrated that the Alg-SLN treated mice showed a higher level of immunity than the mice treated with pure alginate against infections caused by P. aeruginosa. Overally the findings showed the efficacy of new prepared vaccine to induce immunogenicity, and therefore it can be considered as a candidate for a strong vaccine against P. aeruginosa.

铜绿假单胞菌由于其病原体众多且其多重耐药菌株在世界范围内传播而显得尤为重要。因此，需要开发一种有效的疫苗来预防铜绿假单胞菌疾病。本研究的目的是评估藻酸盐 (Alg) 抗原与 SLN 结合作为小鼠模型中针对铜绿假单胞菌的纳米疫苗候选物的免疫原性。海藻酸盐是一种弱免疫原，但海藻酸盐产生的免疫反应可有效杀死假单胞菌。为了提高海藻酸盐的免疫原性，使用了 SLN，它可用于药物输送并可提高长期有效性。 ELISA和调理吞噬试验的结果表明，与单独的藻酸盐抗原相比，Alg-SLN缀合物具有更好的刺激免疫系统产生更多性能更好的免疫球蛋白的能力。攻击测试还表明，与用纯藻酸盐处理的小鼠相比，Alg-SLN处理的小鼠对铜绿假单胞菌引起的感染表现出更高水平的免疫力。总的来说，研究结果表明新制备的疫苗具有诱导免疫原性的功效，因此可以被认为是针对铜绿假单胞菌的强效疫苗的候选者。