Isoniazid and its major metabolite, hydrazine (N₂H₄), may interfere with mitochondrial function and have negative effects on cells. Consequently, an understanding of the role of N₂H₄ in mitochondria is highly desirable for protecting human health. Herein, we report a novel mitochondria-targeted ratiometric fluorescent probe (Mitro-N₂H₄) for N₂H₄ detection. Mitro-N₂H₄ exhibited an attenuation of green emission at 521 nm and an enhancement of yellow emission at 590 nm in the presence of N₂H₄ because of hydrazinolysis, indicating that it can be used as a ratiometric chemosensor for N₂H₄ with high selectivity and sensitivity. Such on-site monitoring of N₂H₄ vapour using test strips and N₂H₄-moistened soil analysis demonstrated its advantages in potential application for the convenient sensing of N₂H₄. Moreover, the rationally designed probe has many potential applications for imaging N₂H₄ produced in situ during the metabolism of isoniazid in living cells based on the ratio of the fluorescent signal.

异烟肼及其主要代谢物肼（N ₂ H ₄）可能会干扰线粒体功能并对细胞产生负面影响。因此，非常需要了解N ₂ H ₄在线粒体中的作用，以保护人类健康。在这里，我们报告了一种新型的针对N ₂ H ₄检测的线粒体靶向比例荧光探针（Mitro-N ₂ H ₄）。在存在N ₂ H ₄的情况下，Mitro-N ₂ H ₄在521 nm处显示绿色发射衰减，在590 nm处显示黄色发射增强。由于肼解作用，表明它可以用作N ₂ H ₄的比例化学传感器，具有很高的选择性和灵敏度。使用试纸和N ₂ H ₄润湿的土壤分析对N ₂ H ₄蒸气进行现场监测，显示了其在方便感测N ₂ H ₄方面的潜在优势。此外，基于荧光信号的比率，合理设计的探针具有许多潜在的应用，可用于在活细胞异烟肼代谢期间原位产生的N ₂ H ₄成像。