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Immunosorbent assay based on upconversion nanoparticles controllable assembly for simultaneous detection of three antibiotics
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2020-11-28 , DOI: 10.1016/j.jhazmat.2020.124703
Yu Wang , Xudong Zhao , Man Zhang , Xuan Sun , Jialei Bai , Yuan Peng , Shuang Li , Dianpeng Han , Shuyue Ren , Jiang Wang , Tie Han , Yifei Gao , Baoan Ning , Zhixian Gao

The abuse of antibiotics leads to an increase in resistant strains, which in turn leads to the development of superbugs that pose great difficulties for the treatment of human diseases. A high-throughput and highly sensitive avidin biotin complex immunosorbent assay based on upconversion nanoparticles controllable assembly (ABC-ULISA) for the detection of antibiotics was developed, which enabled accurate quantitative detection in a shorter period of time. Streptavidin and biotin-labeled upconversion nanoparticles form avidin-biotin-upconversion complex, which was then combined with biotinylated antibody to achieve double amplification of the signal, further improving detection sensitivity. Upconversion nanoparticles with 808 nm excitation provide better penetration without the need for an external source. The 96-well enzyme-linked plate was used as a detection platform to meet the high-throughput needs. ABC-ULISA was used to simultaneously detect three antibiotics with a limit of detection of 0.15 ng/mL for sulfamethazine, 0.03 ng/mL for sarafloxacin, and 0.05 ng/mL for tetracycline. The detection limit of ABC-ULISA was much lower than the traditional ELISA and ordinary ULISA. Moreover, ABC-ULISA was also versatile, and the corresponding target can be detected by changing different antibodies. The results were stable and reliable, and the equipment could be miniaturized, which was expected to be commercialized and on-site.



中文翻译:

基于上转换纳米颗粒可控组件的免疫吸附测定,可同时检测三种抗生素

抗生素的滥用导致耐药菌株的增加,进而导致超级细菌的发展,而超级细菌对人类疾病的治疗造成很大困难。开发了一种基于上转换纳米颗粒可控组件(ABC-ULISA)的高通量,高灵敏度的抗生物素蛋白生物素复合物免疫吸附剂检测抗生素的方法,该方法能够在较短的时间内进行准确的定量检测。链霉亲和素和生物素标记的上转换纳米颗粒形成抗生物素蛋白-生物素上转换复合物,然后与生物素化抗体结合以实现信号的双重放大,从而进一步提高检测灵敏度。具有808 nm激发的上转换纳米颗粒无需外部源即可提供更好的穿透力。96孔酶联反应板用作检测平台,可满足高通量需求。ABC-ULISA用于同时检测三种抗生素,对磺胺二甲嘧啶的检出限为0.15 ng / mL,对沙拉沙星的检出限为0.03 ng / mL,对四环素的检出限为0.05 ng / mL。ABC-ULISA的检出限远低于传统ELISA和普通ULISA。此外,ABC-ULISA还是通用的,可以通过更换不同的抗体来检测相应的靶标。结果稳定可靠,设备可以小型化,有望实现商业化和现场化。四环素的浓度为05 ng / mL。ABC-ULISA的检出限远低于传统ELISA和普通ULISA。此外,ABC-ULISA还是通用的,可以通过更换不同的抗体来检测相应的靶标。结果稳定可靠,设备可以小型化,有望实现商业化和现场化。四环素的浓度为05 ng / mL。ABC-ULISA的检出限远低于传统ELISA和普通ULISA。此外,ABC-ULISA还是通用的,可以通过更换不同的抗体来检测相应的靶标。结果稳定可靠,设备可以小型化,有望实现商业化和现场化。

更新日期:2020-12-08
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