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Morphology and ontogenesis of two new Hemiholosticha species (Ciliophora, Hypotrichia, Hemiholostichidae nov. fam.)
European Journal of Protistology ( IF 1.9 ) Pub Date : 2020-11-30 , DOI: 10.1016/j.ejop.2020.125763
Peter Vďačný , Wilhelm Foissner

The morphology and ontogenesis of two new hypotrich ciliates, Hemiholosticha solitaria and Hemiholosticha germanica, were studied using live observation, protargol impregnation, and scanning electron microscopy. Both species share a medium-sized, almost globular body with a short anterior projection; two macronuclear nodules with a single micronucleus in between; a central contractile vacuole; three or four ventral, one postoral, one right and one left marginal cirral row; and three dorsal kineties extending along ribs. However, H. germanica is distinguished from congeners by a higher number of cirri in ventral rows R1 and R2 (3–6 vs. 2 cirri in each row). Hemiholosticha solitaria differs from congeners by having four (vs. three) ventral cirral rows and by the lack (vs. presence) of intracellular green algae. The ontogenesis of H. solitaria follows the H. pantanalensis mode in that (i) the oral primordium develops in a deep pouch and generates the first two cirral streaks in addition to adoral membranelles and undulating membranes, (ii) the undulating membrane anlage does not produce any cirri, and (iii) the longitudinal ventral cirral row R3 originates from two anlagen. The ontogenetic peculiarities along with the 18S rRNA gene phylogenies suggest classification of Hemiholosticha, Psilotrichides, and Urospinula into a new family, Hemiholostichidae.



中文翻译:

两种半球形新物种(Ciliophora,Hypotrichia,Hemiholostichidae nov。fam。)的形态和成因

使用实时观察,普萘塔尔浸渍和扫描电子显微镜研究了两种新的伪毛纤毛虫(Hemiholosticha solitariaHemiholosticha germanica)的形态和致瘤作用。这两个物种共享一个中等大小的,几乎呈球形的身体,前部投影较短。两个大核结节,中间有一个微核;中央收缩液泡; 三或四个腹侧,一个邮政,右边缘和左边缘一排。和三个背肋沿肋骨延伸。但是,德国猪血吸虫与同类动物的区别在于腹侧R1和R2中的卷毛数量较多(每行3-6个卷毛与2个卷毛)。孤草与同类物的区别在于,腹侧有四排(相对于三排),而胞内绿藻则缺乏(与存在)。的个体发生H. solitaria跟随H. pantanalensis在该模式(i)所述口服原基在深袋开发和除了奥多拉尔小膜和起伏产生的膜的前两个cirral条纹,(ii)所述波动膜原基不产生任何卷毛,(iii)腹侧纵行R3源自两个anlagen。与18S rRNA基因系统发育一起的个体发育特点表明,将半壳纲PsilotrichidesUrospinula分为一个新家族,半壳纲。

更新日期:2020-12-08
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