Derivatives of poly(styrene-co-maleic acid) (pSMA), have recently emerged as effective reagents for extracting membrane protein complexes from biological membranes. Despite recent progress in using SMAs to study artificial and bacterial membranes, very few reports have addressed their use in studying the highly abundant and well characterized thylakoid membranes. Recently, we tested the ability of twelve commercially available SMA copolymers with different physicochemical properties to extract membrane protein complexes (MPCs) from spinach thylakoid membrane. Based on the efficacy of both protein and chlorophyll extraction, we have found five highly efficient SMA copolymers: SMA® 1440, XIRAN® 25010, XIRAN® 30010, SMA® 17352, and SMA® PRO 10235, that show promise in extracting MPCs from chloroplast thylakoids. To further advance the application of these polymers for studying biomembrane organization, we have examined the composition of thylakoid supramolecular protein complexes extracted by the five SMA polymers mentioned above. Two commonly studied plants, spinach (Spinacia oleracea) and pea (Pisum sativum), were used for extraction as model biomembranes. We found that the pSMAs differentially extract protein complexes from spinach and pea thylakoids. Based on their differential activity, which correlates with the polymer chemical structure, pSMAs can be divided into two groups: unfunctionalized polymers and ester derivatives.

聚（苯乙烯-马来酸）（pSMA）衍生物最近已成为从生物膜中提取膜蛋白复合物的有效试剂。尽管最近在使用 SMA 研究人造膜和细菌膜方面取得了进展，但很少有报告讨论它们在研究高度丰富且特征明确的类囊体膜中的用途。最近，我们测试了 12 种具有不同理化性质的市售 SMA 共聚物从菠菜类囊体膜中提取膜蛋白复合物 (MPC) 的能力。基于蛋白质和叶绿素提取的功效，我们发现了五种高效 SMA 共聚物：SMA® 1440、XIRAN® 25010、XIRAN® 30010、SMA® 17352 和 SMA® PRO 10235，它们有望从叶绿体中提取 MPC类囊体。为了进一步推进这些聚合物在生物膜组织研究中的应用，我们研究了由上述五种SMA聚合物提取的类囊体超分子蛋白复合物的组成。两种常用的研究植物，菠菜（ Spinacia oleracea ）和豌豆（ Pisum sativum ），被用作模型生物膜进行提取。我们发现 pSMA 从菠菜和豌豆类囊体中提取蛋白质复合物有差异。根据与聚合物化学结构相关的不同活性，pSMA 可分为两类：非功能化聚合物和酯衍生物。