Multiphoton microscopy (MPM) can create high penetration depth, low photodamage, real-time and 3-dimensional-tissue subcellular resolution images of the cornea. It surmounts the difficulty of non-destructive or in vivo detection caused by the sensitivity of the cornea. The emerging technology overcomes the high photodamage and low image contrast of general optical inspection by using nonlinear optical processes. Different chemical composition in the cornea excites diverse up-conversion photons to form the image and spectra, providing accurate and comprehensive information of the cornea. MPM, which combines the advantages of optical imaging and spectral measurement technique, provides the detection of metabolic, cell density, keratitis, drug delivery, corneal thickness, intraocular pressure, and fiber orientation of the cornea. We review the state of the art of MPM imaging analysis studies and discuss prospects.

多光子显微镜（MPM）可以创建角膜的高穿透深度，低光损伤，实时和3维组织的亚细胞分辨率图像。它克服了非破坏性或体内的困难检测是由角膜的敏感性引起的。新兴技术通过使用非线性光学工艺，克服了常规光学检测的高光损伤和低图像对比度的问题。角膜中不同的化学成分激发各种上转换光子以形成图像和光谱，从而提供了准确而全面的角膜信息。MPM结合了光学成像和光谱测量技术的优势，可检测代谢，细胞密度，角膜炎，药物输送，角膜厚度，眼内压和角膜纤维方向。我们回顾了MPM成像分析研究的最新发展并讨论了前景。